Construction of scFv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library

2010 ◽  
Vol 231 (3) ◽  
pp. 423-430 ◽  
Author(s):  
Xiao Zhang ◽  
Cunzheng Zhang ◽  
Yuan Liu ◽  
Xiangyang Yu ◽  
Xianjin Liu
Keyword(s):  
Phage Display ◽  
Phage Display Library

Selection and Characterization of Human Serum Albumin-specific Porcine scFv Antibodies Using a Phage Display Library

2014 ◽  
Vol 33 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Junko Muraoka ◽  
Takuya Ozawa ◽  
Yurie Enomoto ◽  
Norihiko Kiyose ◽  
Ayana Imamura ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Phage Display ◽  
Human Serum ◽  
Phage Display Library

scholarly journals Isolation of and Characterization of Neutralizing Antibodies to Covid-19 from a Large Human Naïve scFv Phage Display Library

2020 ◽  
Author(s):  
Andy Q. Yuan ◽  
Likun Zhao ◽  
Lili Bai ◽  
Qingwu Meng ◽  
Zhenguo Wen ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Epitope Mapping ◽  
Neutralization Assay ◽  
Phage Display Library ◽  
Antibody Library ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Human Receptor

AbstractSARS-CoV-2 (Covid-19) has caused currently ongoing global plague and imposed great challenges to health managing systems all over the world, with millions of infections and hundreds of thousands of deaths. In addition to racing to develop vaccines, neutralizing antibodies (nAbs) to this virus have been extensively sought and are expected to provide another prevention and therapy tool against this frantic pandemic. To offer fast isolation and shortened early development, a large human naïve phage display antibody library, was built and used to screen specific nAbs to the receptor-binding domain, RBD, the key for Covid-19 virus entry through a human receptor, ACE2. The obtained RBD-specific antibodies were characterized by epitope mapping, FACS and neutralization assay. Some of the antibodies demonstrated spike-neutralizing property and ACE2-competitiveness. Our work proved that RBD-specific neutralizing binders from human naïve antibody phage display library are promising candidates to for further Covid-19 therapeutics development.

scholarly journals Characterization of an anti-RNA recombinant autoantibody fragment (scFv) isolated from a phage display library and detailed analysis of its binding site on U1 snRNA

RNA ◽  
1998 ◽  
Vol 4 (9) ◽  
pp. 1124-1133 ◽  
Author(s):  
S.W.M. TEUNISSEN ◽  
M.H.W. STASSEN ◽  
G.J.M. PRUIJN ◽  
W.J. VAN VENROOIJ ◽  
R.M.A. HOET
Keyword(s):  
Phage Display ◽  
Binding Site ◽  
Detailed Analysis ◽  
Phage Display Library ◽  
U1 Snrna

Characterization of human blood group scFv antibodies derived from a V gene phage-display library

1994 ◽  
Vol 88 (1) ◽  
pp. 180-186 ◽  
Author(s):  
N. C. Hughes-Jones ◽  
B. D. Gorick ◽  
J. M. Bye ◽  
R. Finnern ◽  
M. L. Scott ◽  
...  
Keyword(s):  
Phage Display ◽  
Blood Group ◽  
Human Blood ◽  
Phage Display Library ◽  
Human Blood Group ◽  
V Gene
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