scholarly journals Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

2001 ◽  
Vol 39 (2) ◽  
pp. 109-116 ◽  
Author(s):  
H. Daniell ◽  
B. Muthukumar ◽  
S. B. Lee
Keyword(s):  
Transgenic Plants ◽  
Chloroplast Genome ◽  
Antibiotic Selection ◽  
Marker Free

Production of Marker-Free Transgenic Plants

2021 ◽  
pp. 223-251
Author(s):  
Urvashi Sharma ◽  
Ajinder Kaur ◽  
Jagdeep Singh Sandhu
Keyword(s):  
Transgenic Plants ◽  
Marker Free

Recent Developments in Generation of Marker-Free Transgenic Plants

2019 ◽  
pp. 127-142
Author(s):  
Rupesh Kumar Singh ◽  
Lav Sharma ◽  
Nitin Bohra ◽  
Sivalingam Anandhan ◽  
Eliel Ruiz-May ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Recent Developments ◽  
Marker Free

scholarly journals A new double right border binary vector for producing marker-free transgenic plants

2013 ◽  
Vol 6 (1) ◽  
pp. 448 ◽  
Author(s):  
Jonathan M Matheka ◽  
Sylvester Anami ◽  
James Gethi ◽  
Rasha A Omer ◽  
Amos Alakonya ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Binary Vector ◽  
Right Border ◽  
Marker Free

scholarly journals Marker-Free Transgenic Plants through Genetically Programmed Auto-Excision

2007 ◽  
Vol 145 (4) ◽  
pp. 1220-1231 ◽  
Author(s):  
Dimitri Verweire ◽  
Kristof Verleyen ◽  
Sylvie De Buck ◽  
Martine Claeys ◽  
Geert Angenon
Keyword(s):  
Transgenic Plants ◽  
Marker Free

Transformation Systems for Generating Marker–Free Transgenic Plants

1994 ◽  
Vol 12 (3) ◽  
pp. 263-267 ◽  
Author(s):  
John I. Yoder ◽  
Andrew P. Goldsbrough
Keyword(s):  
Transgenic Plants ◽  
Marker Free ◽  
Transformation Systems

Selection of Marker-Free Transgenic Plants Using the Oncogenes (ipt, rol A, B, C) of Agrobacterium as Selectable Markers

2000 ◽  
pp. 25-46 ◽  
Author(s):  
Hiroyasu Ebinuma ◽  
Koichi Sugita ◽  
Etsuko Matunaga ◽  
Saori Endo ◽  
Takehide Kasahara
Keyword(s):  
Transgenic Plants ◽  
Selectable Markers ◽  
Marker Free ◽  
Selection Of

A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency

2000 ◽  
Vol 22 (5) ◽  
pp. 461-469 ◽  
Author(s):  
Koichi Sugita ◽  
Takehide Kasahara ◽  
Etsuko Matsunaga ◽  
Hiroyasu Ebinuma
Keyword(s):  
Transgenic Plants ◽  
High Frequency ◽  
Single Copy ◽  
Transformation Vector ◽  
Marker Free

A novel double T-DNA system for producing stack and marker-free transgenic plants

2016 ◽  
Author(s):  
X. J. Wang ◽  
Y. Y. Su ◽  
Y. F. Dong ◽  
Q. L. Tang ◽  
Z. X. Wang
Keyword(s):  
Transgenic Plants ◽  
Marker Free

Stable transformation ofphaC2 gene in tobacco chloroplast genome

2006 ◽  
Vol 3 (3) ◽  
pp. 201-207
Author(s):  
Wang Yu-Hua ◽  
Wu Zhong-Yi ◽  
Zhang Xiu-Hai ◽  
Wang Yong-Qin ◽  
Huang Cong-Lin ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Chloroplast Genome ◽  
Marker Gene ◽  
Chloroplast Transformation ◽  
Single Copy ◽  
Stable Transformation ◽  
Pha Synthase ◽  
Type Ii ◽  
Tobacco Chloroplast ◽  
Chloroplast Genomes

AbstractMedium-chain-length polyhydroxyalkanoates (mcl-PHAs) belong to the group of microbial polyesters. The key enzyme for mcl-PHA biosynthesis is type II PHA synthase. The genephaC2 encoding type II PHA synthase was placed under the control ofpsbA-pro andpsbA-ter of rice (Oryza sativa) to construct aphaC2 cassette, which was ligated with the screening marker geneaadAcassette (prrn–aadA–TpsbA-ter). These recombined fragments were cloned between the plastidrbcLandaccDgenes for targeting to the large single copy region of the chloroplast genome. A chloroplast transformation vector, pTC2, was constructed and introduced into the tobacco (Nicotiana tobacum) chloroplast genome by particle bombardment. PCR and Southern blot analysis confirmed stable integration ofphaC2 into the chloroplast genomes of T0and T1transgenic plants, and T1transgenic plants exhibited homoplasmy. The expression ofphaC2 at transcription level was detected by reverse transcriptase–polymerase chain reaction (RT-PCR). Recombinant transgenes in the tobacco chloroplast genome were maternally inherited and were not transmitted via pollen when out-crossed with untransformed female plants. To our knowledge, this is the first report on the stable transformation ofphaC2 encoding type II PHA synthase in tobacco via chloroplast genetic engineering.

scholarly journals Selection of marker-free transgenic plants using the isopentenyl transferase gene

1997 ◽  
Vol 94 (6) ◽  
pp. 2117-2121 ◽  
Author(s):  
H. Ebinuma ◽  
K. Sugita ◽  
E. Matsunaga ◽  
M. Yamakado
Keyword(s):  
Transgenic Plants ◽  
Isopentenyl Transferase ◽  
Transferase Gene ◽  
Marker Free ◽  
Selection Of
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