Molecular cloning, functional expression and characterization of two serine/threonine-specific protein kinases from Nicotiana tabacum pollen

2004 ◽  
Vol 17 (4) ◽  
pp. 165-175 ◽  
Author(s):  
Kumara Dissanayake ◽  
Carlos Castillo ◽  
Takeshi Takasaki ◽  
Tetsu Nakanishi ◽  
Naoko Norioka ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Molecular Cloning ◽  
Protein Kinases ◽  
Functional Expression ◽  
Specific Protein

scholarly journals Characterization of an activated human ros gene.

1986 ◽  
Vol 6 (9) ◽  
pp. 3109-3116 ◽  
Author(s):  
C Birchmeier ◽  
D Birnbaum ◽  
G Waitches ◽  
O Fasano ◽  
M Wigler
Keyword(s):  
Gene Transfer ◽  
Protein Kinases ◽  
Transmembrane Domain ◽  
Extracellular Domain ◽  
Kinase Domain ◽  
Specific Protein ◽  
Normal Human

A human oncogene, mcf3, previously detected by a combination of DNA-mediated gene transfer and a tumorigenicity assay, derives from a human homology of the avian v-ros oncogene. Both v-ros and mcf3 can encode a protein with homology to tyrosine-specific protein kinases, and both mcf3 and v-ros encode a potential transmembrane domain N terminal to the kinase domain. mcf3 probably arose during gene transfer from a normal human ros gene by the loss of a putative extracellular domain. There do not appear to be any other gross rearrangements in the structure of mcf3.

Arabidopsis homologs of the shaggy and GSK-3 protein kinases: molecular cloning and functional expression in Escherichia coli

1994 ◽  
Vol 242 (3) ◽  
pp. 337-345 ◽  
Author(s):  
Michele W. Bianchi ◽  
Dominique Guivarc'h ◽  
Martine Thomas ◽  
James R. Woodgett ◽  
Martin Kreis
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Protein Kinases ◽  
Functional Expression ◽  
Expression In Escherichia Coli

scholarly journals Molecular cloning and characterization of cDNA coding for  1,2N-acetylglucosaminyltransferase I (GlcNAc-TI) from Nicotiana tabacum

Glycobiology ◽  
1999 ◽  
Vol 9 (8) ◽  
pp. 779-785 ◽  
Author(s):  
R. Strasser ◽  
J. Mucha ◽  
H. Schwihla ◽  
F. Altmann ◽  
J. Glossl ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Molecular Cloning

Molecular cloning, functional expression and characterization of p15, a novel fungal protein with potent neurite-inducing activity in PC12 cells

2001 ◽  
Vol 1522 (2) ◽  
pp. 74-81 ◽  
Author(s):  
Shuji Wakatsuki ◽  
Tatsuya Yokoyama ◽  
Satoru Nakashima ◽  
Akiyoshi Nishimura ◽  
Manabu Arioka ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Pc12 Cells ◽  
Functional Expression ◽  
Fungal Protein

scholarly journals Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta

1997 ◽  
Vol 328 (3) ◽  
pp. 739-743 ◽  
Author(s):  
Mark GRIFFITHS ◽  
Y. M. Sylvia YAO ◽  
Fatima ABIDI ◽  
E. V. Simon PHILLIPS ◽  
E. Carol CASS ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Human Placenta ◽  
Ovarian Tissue ◽  
Functional Expression ◽  
Nucleoside Transporter ◽  
Intact Cells ◽  
Equilibrative Nucleoside Transporter ◽  
Equilibrative Nucleoside Transporters ◽  
Sequence Deletion

Mammalian equilibrative nucleoside transporters are typically divided into two classes, es and ei, based on their sensitivity or resistance respectively to inhibition by nitrobenzylthioinosine (NBMPR). Previously, we have reported the isolation of a cDNA clone encoding a prototypic es-type transporter, hENT1 (human equilibrative nucleoside transporter 1), from human placenta. We now report the molecular cloning and functional expression in Xenopus oocytes of a cDNA from the same tissue encoding a homologous ei-type transporter, which we designate hENT2. This 456-residue protein is 46% identical in amino acid sequence with hENT1 and corresponds to a full-length form of the delayed-early proliferative response gene product HNP36, a protein of unknown function previously cloned in a form bearing a sequence deletion. In addition to placenta, hENT2 is found in brain, heart and ovarian tissue. Like hENT1, hENT2 mediates saturable transport of the pyrimidine nucleoside uridine (Km 0.2±0.03 mM) and also transports the purine nucleoside adenosine. However, in contrast with hENT1, which is potently inhibited by NBMPR (Ki 2 nM), hENT2 is NBMPR-insensitive (IC50 < 1 μM). It is also much less sensitive to inhibition by the coronary vasoactive drugs dipyridamole and dilazep and to the lidoflazine analogue draflazine, properties that closely resemble those reported for classical ei-type transport in studies with intact cells.

scholarly journals Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Schizonepeta tenuifolia.

2001 ◽  
Vol 24 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Takuro MARUYAMA ◽  
Michiho ITO ◽  
Fumiyuki KIUCHI ◽  
Gisho HONDA
Keyword(s):  
Molecular Cloning ◽  
Functional Expression ◽  
Limonene Synthase ◽  
Schizonepeta Tenuifolia

scholarly journals Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum

1996 ◽  
Vol 37 (3) ◽  
pp. 369-376 ◽  
Author(s):  
Y. Y. Setiady ◽  
M. Sekine ◽  
N. Hariguchi ◽  
H. Kouchi ◽  
A. Shinmyo
Keyword(s):  
Nicotiana Tabacum ◽  
Molecular Cloning ◽  
Cdna Clone
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