Peptide from glutamic acid decarboxylase similar to coxsackie B virus stimulates IFN- γ mRNA expression in Th1-like lymphocytes from children with recent-onset insulin-dependent diabetes mellitus

1998 ◽  
Vol 35 (3) ◽  
pp. 137-144 ◽  
Author(s):  
M. G. E. Karlsson ◽  
J. Ludvigsson
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
Acid Decarboxylase ◽  
Recent Onset ◽  
B Virus ◽  
Coxsackie B Virus ◽  
Insulin Dependent ◽  
Ifn Γ ◽  
Coxsackie B

scholarly journals Fusion Proteins for Combined Analysis of Autoantibodies to the 65-kDa Isoform of Glutamic Acid Decarboxylase and Islet Antigen-2 in Insulin-dependent Diabetes Mellitus

2001 ◽  
Vol 47 (5) ◽  
pp. 926-934 ◽  
Author(s):  
Mathias Rickert ◽  
Jochen Seissler ◽  
Werner Dangel ◽  
Helga Lorenz ◽  
Wiltrud Richter
Keyword(s):  
Diabetes Mellitus ◽  
Fusion Protein ◽  
Cost Effective ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Acid Decarboxylase ◽  
Immune Reactivity ◽  
Insulin Dependent ◽  
Islet Antigen

Abstract Background: Prediction, risk assessment, and diagnosis of autoimmune diseases often rely on detection of autoantibodies directed to multiple target antigens, such as the 65-kDa isoform of glutamic acid decarboxylase (GAD65-abs) and the tyrosine phosphatase-like protein islet antigen-2 (IA2-abs), the two major subspecificities of islet cell antibodies (ICAs) associated with insulin-dependent diabetes mellitus. We hypothesized that a combination of autoantigens in a fusion protein unifying the important immunodominant epitopes could provide an efficient target for cost-effective, one-step screening of sera. Methods: Chimeric proteins composed of GAD65 and IA2 residues were constructed, analyzed for their immune reactivity with monoclonal antibodies and sera, and used in a diagnostic assay with 35S-labeled protein as antigen. Results: Length and order of GAD65 and IA2 sequences were critical for conservation of the conformational epitopes in the fusion protein. Among four chimera tested, only IA2(606–979)/GAD65(1–585) retained wild-type-like folding of GAD65 and IA2 domains and yielded a stable protein after baculovirus expression. Reactivity of GAD65 antibody- and IA2 antibody-positive sera from patients newly diagnosed with insulin-dependent diabetes mellitus, from ICA-positive prediabetics, and from ICA-positive first-degree relatives demonstrated conservation of the relevant autoreactive epitopes. The assay based on the in vitro translated fusion antigen had a sensitivity and specificity identical to those for detection of GAD65- and IA2-abs based on the two separate GAD65 and IA2 proteins. Conclusions: Autoantigens such as GAD65 and IA2 can be combined successfully in a fusion protein of similar immune reactivity. This allows simultaneous detection of GAD65- and IA2-abs in a one-step screening assay and cost-effective identification of positive individuals at risk of diabetes or at onset of disease.

Effects of cyclosporine immunosuppression in insulin-dependent diabetes mellitus of recent onset

Science ◽  
1984 ◽  
Vol 223 (4643) ◽  
pp. 1362-1367 ◽  
Author(s):  
C. Stiller ◽  
J Dupre ◽  
M Gent ◽  
M. Jenner ◽  
P. Keown ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Recent Onset ◽  
Insulin Dependent
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