Biological and molecular characterization of a tomato isolate of potato virus Y (PVY) of the PVYC lineage

2016 ◽  
Vol 161 (12) ◽  
pp. 3561-3566 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Dalton Vander Pol ◽  
Olga V. Nikolaeva ◽  
Michael J. Melzer ◽  
Alexander V. Karasev
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Tomato Isolate

Distribution and molecular characterization of potato virus Y (PVY) strains infecting potato (Solanum tuberosum) crop in Kashmir (India)

VirusDisease ◽  
2021 ◽  
Author(s):  
Sumiah Wani ◽  
Sahar Saleem ◽  
Sajad U. Nabi ◽  
Gowhar Ali ◽  
Bilal A. Paddar ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

scholarly journals Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y

2016 ◽  
Vol 60 (04) ◽  
pp. 347-353 ◽  
Author(s):  
N. SIHELSKÁ ◽  
L. PREDAJŇA ◽  
A. NAGYOVÁ ◽  
K. ŠOLTYS ◽  
J. BUDIŠ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Recombinant Strains

Molecular characterization of potato virus Y resistance in potato (Solanum tuberosumL.)

2015 ◽  
Vol 75 (3) ◽  
pp. 389 ◽  
Author(s):  
Vinay Bhardwaj ◽  
Reena Sharma ◽  
Dalamu ◽  
A. K. Srivastava ◽  
R. Baswaraj ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

scholarly journals Identification and Molecular Characterization of Recombinant Potato Virus Y (PVY) in Potato from South Korea, PVYNTN Strain

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Mariana Rodriguez-Rodriguez ◽  
Kelsie J. Green ◽  
Dong-Jun Kim ◽  
Sang-Min Chung ◽  
...  
Keyword(s):  
South Korea ◽  
Whole Genome Sequencing ◽  
Molecular Characterization ◽  
Genome Sequencing ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Production ◽  
Whole Genome ◽  
Rt Pcr

Potato is an important source of food in South Korea, and viruses represent a significant threat to sustainable and profitable potato production. However, information about viruses affecting the potato crop in South Korea is limited. In 2017, potato plants of five cultivars exhibiting foliar mosaic, crinkling, and mottle were collected in two seed potato production areas, in Gangwon-do and Jeollabuk-do Provinces, and subjected to virus testing and characterization. Potato virus Y (PVY) was found associated with mosaic symptoms, and samples were characterized using reverse transcription polymerase chain reaction (RT-PCR) and whole genome sequencing. All analyzed PVY-positive samples were found to represent the same recombinant PVY strain: PVYNTN. Three PVY isolates were subjected to whole genome sequencing using overlapping RT-PCR fragments and Sanger methodology, and all three were confirmed to represent strain PVYNTNa after a recombination analysis of the complete genomes. In phylogenetic analysis, the three South Korean isolates were placed most closely to several PVYNTNa isolates reported from Japan and Vietnam, suggesting a common source of infection. This is the first report and complete molecular characterization of a PVYNTN strain present in the country, and because this strain induces tuber necrotic ringspot disease in susceptible cultivars of potato, appropriate management tools need to be implemented to mitigate potential tuber quality losses.

Biological and Molecular Characterization of Potato virus Y Infecting Potato (Solanum tuberosum) in India

2013 ◽  
Vol 6 (5) ◽  
pp. 257-264 ◽  
Author(s):  
Pooja Sharma ◽  
Anurag Kumar Sahu ◽  
Rakesh Kumar Verma ◽  
Ritesh Mishra ◽  
R.K. Gaur
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Biological and molecular characterization of two tomato strains of potato virus Y (PVY)

2000 ◽  
Vol 22 (3) ◽  
pp. 336-343 ◽  
Author(s):  
C. Morel ◽  
P. Gognalons ◽  
L. Guilbaud ◽  
C. Caranta ◽  
K. Gébré-Sélassié ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Molecular characterization of two recombinant potato virus Y isolates from China

2011 ◽  
Vol 157 (2) ◽  
pp. 401-403 ◽  
Author(s):  
Biao Wang ◽  
Jin-Lei Jia ◽  
Xiao-Qiang Wang ◽  
Zhen-Yu Wang ◽  
Bing-Hui Yang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

2005 ◽  
Vol 125 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Emmanuel Jacquot ◽  
Michel Tribodet ◽  
Flora Croizat ◽  
Valérie Balme-Sinibaldi ◽  
Camille Kerlan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Sign in / Sign up

Export Citation Format

Share Document