Molecular cloning, expression, and characterization of a thermostable glutamate racemase from a hyperthermophilic bacterium, Aquifex pyrophilus

Extremophiles ◽  
1999 ◽  
Vol 3 (3) ◽  
pp. 175-183 ◽  
Author(s):  
Sang Suk Kim ◽  
I.-G. Choi ◽  
Sung-Hou Kim ◽  
Y. G. Yu
Keyword(s):  
Molecular Cloning ◽  
Glutamate Racemase ◽  
Hyperthermophilic Bacterium ◽  
Aquifex Pyrophilus

Molecular cloning and characterization of thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic bacterium

Extremophiles ◽  
2001 ◽  
Vol 5 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Jae-Hwan Lim ◽  
Juhyun Choi ◽  
Soo-Jin Han ◽  
Sung Kim ◽  
Hye-Zin Hwang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Dna Ligase ◽  
Hyperthermophilic Bacterium ◽  
Aquifex Pyrophilus

Crystallization and preliminary X-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium

1999 ◽  
Vol 55 (4) ◽  
pp. 927-928 ◽  
Author(s):  
Kwang Yeon Hwang ◽  
Chun-Seok Cho ◽  
Sang Suk Kim ◽  
Kyuwon Baek ◽  
Sung-Hou Kim ◽  
...  
Keyword(s):  
Diffusion Method ◽  
Data Sets ◽  
Hanging Drop ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Glutamate Racemase ◽  
Hyperthermophilic Bacterium ◽  
Polyethylene Glycol 6000 ◽  
Aquifex Pyrophilus

Glutamate racemase catalyzes the reversible reaction of L-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with unit-cell parameters a = b = 72.1, c = 185.02 Å. The asymmetric unit contains one molecule, corresponding to a Vm value of 2.35 Å3 Da−1. Complete data sets from a native and a mercury-derivative crystal have been collected at 2.0 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.

Molecular cloning and characterization of vernalization-related (ver) genes in winter wheat

1994 ◽  
Vol 92 (3) ◽  
pp. 511-515 ◽  
Author(s):  
Kang Chong ◽  
Li-Ping Wang ◽  
Ke-Hui Tan ◽  
Hua-Liang Huang ◽  
Hou-Guo Liang
Keyword(s):  
Winter Wheat ◽  
Molecular Cloning

Molecular cloning and characterization of calcineurin A in Setosphaeria turcica

2009 ◽  
Vol 31 (10) ◽  
pp. 1059-1064
Author(s):  
Zhi-Yong LI ◽  
Zhi-Min HAO ◽  
Zhi-Ping DONG ◽  
He-Long SI ◽  
Jin-Gao DONG
Keyword(s):  
Molecular Cloning ◽  
Setosphaeria Turcica ◽  
Calcineurin A

Molecular Cloning and Characterization of Nitrite Reductase Gene from Sugarbeet

2011 ◽  
Vol 37 (8) ◽  
pp. 1406-1414
Author(s):  
Xiao-Yan SHI ◽  
Yan-Da ZENG ◽  
Shi-Long LI ◽  
Yu-Bo WANG ◽  
Feng-Ming MA ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Nitrite Reductase ◽  
Nitrite Reductase Gene ◽  
Reductase Gene
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