Cloning, expression, and biochemical characterization of 3-deoxy-d- manno -2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus

Extremophiles ◽  
2003 ◽  
Vol 7 (6) ◽  
pp. 471-481 ◽  
Author(s):  
Smadar Shulami ◽  
Orit Yaniv ◽  
Emilia Rabkin ◽  
Yuval Shoham ◽  
Timor Baasov
Keyword(s):  
Biochemical Characterization ◽  
Hyperthermophilic Bacterium ◽  
Aquifex Pyrophilus

Molecular cloning and characterization of thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic bacterium

Extremophiles ◽  
2001 ◽  
Vol 5 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Jae-Hwan Lim ◽  
Juhyun Choi ◽  
Soo-Jin Han ◽  
Sung Kim ◽  
Hye-Zin Hwang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Dna Ligase ◽  
Hyperthermophilic Bacterium ◽  
Aquifex Pyrophilus

scholarly journals Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima

2003 ◽  
Vol 370 (2) ◽  
pp. 651-659 ◽  
Author(s):  
Leon D. KLUSKENS ◽  
Gert-Jan W.M. van ALEBEEK ◽  
Alphons G.J. VORAGEN ◽  
Willem M. de VOS ◽  
John van der OOST
Keyword(s):  
Thermal Inactivation ◽  
Biochemical Characterization ◽  
Thermotoga Maritima ◽  
Pectate Lyase ◽  
Major Product ◽  
Degree Of Polymerization ◽  
Extracellular Medium ◽  
Native Form ◽  
Hyperthermophilic Bacterium

The ability of the hyperthermophilic bacterium Thermotoga maritima to grow on pectin as a sole carbon source coincides with the secretion of a pectate lyase A (PelA) in the extracellular medium. The pelA gene of T. maritima was functionally expressed in Escherichia coli as the first heterologously produced thermophilic pectinase, and purified to homogeneity. Gel filtration indicated that the native form of PelA is tetrameric. Highest activity (422units/mg, with a Km of 0.06mM) was demonstrated on polygalacturonic acid (PGA), whereas pectins with an increasing degree of methylation were degraded at a decreasing rate. In the tradition of pectate lyases, PelA demonstrated full dependency on Ca2+ for stability and activity. The enzyme is highly thermoactive and thermostable, operating optimally at 90°C and pH9.0, with a half-life for thermal inactivation of almost 2h at 95°C, and an apparent melting temperature of 102.5°C. Detailed characterization of the product formation with PGA indicated that PelA has a unique eliminative exo-cleavage pattern liberating unsaturated trigalacturonate as the major product, in contrast with unsaturated digalacturonate for other exopectate lyases known. The unique exo-acting mode of action was supported by progression profiles of PelA on oligogalacturonides (degree of polymerization, 3—8) and the examination of the bond cleavage frequencies.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

Interaction effect of rootstocks on gas exchange parameters, biochemical changes and nutrientstatus in Sauvignon Blanc winegrapes

2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke
Keyword(s):  
Biochemical Characterization ◽  
Reducing Sugar ◽  
Wine Grape ◽  
Gas Exchange Parameters ◽  
Food Material ◽  
Total Carbohydrates ◽  
Salt Creek ◽  
Grape Varieties ◽  
Photosynthetic Activities

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.

Morphological and biochemical characterization of rhizobacteria associated with peach plant and evaluation of these rhizobacteria against its fungal pathogens

2020 ◽  
Vol 52 (6) ◽  
Author(s):  
M. Inam Ul Haq ◽  
Syed Afraz Ali ◽  
Gulshan Irshad ◽  
M Azam Khan ◽  
Shoaib Ismail
Keyword(s):  
Fungal Pathogens ◽  
Biochemical Characterization
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