Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib

2007 ◽  
Vol 51 (3) ◽  
pp. 514-516 ◽  
Author(s):  
A. Subotic ◽  
D. Grubisic
Keyword(s):  
Somatic Embryogenesis ◽  
Histological Analysis ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Root Explants ◽  
Adventitious Shoot Formation

scholarly journals Plantlet formation in black and white spruce. II. Histological analysis of adventitious shoot formation in vitro

1986 ◽  
Vol 64 (5) ◽  
pp. 997-1002 ◽  
Author(s):  
Colin Rumary ◽  
Kamlesh R. Patel ◽  
Trevor A. Thorpe
Keyword(s):  
Histological Analysis ◽  
De Novo ◽  
Vascular System ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
White Spruce ◽  
Shoot Formation ◽  
Intense Staining ◽  
Adventitious Shoot Formation ◽  
Black And White

Histological analysis of developmental events during adventitious shoot formation in cultured epicotyl explants of black and white spruce was carried out. Cells towards the periphery of the expiants and between the bases of the cotyledons responded to cytokinin and became meristematic. Cell division in these regions led to the formation of meristemoids and ultimately intercotyledonary adventitious shoots. These shoots developed a vascular system, which became connected to the main vascular system of the expiant. After about a month of culture, the entire expiant became meristematic and showed evidence of additional de novo shoot development. Several rings of bud primordia were found at the periphery of the expiant after about 40 days of culture. These primordia that developed in the subepidermal layers could easily be distinguished from the surrounding cells by their distinct boundaries and intense staining for nucleic acids and proteins. After 2 months of culture, the entire expiant was covered with well-developed shoots having organized apical meristems, needle primordia, and vascular systems.

Adventitious shoot formation in tulip: histological analysis and response to selective agents

Plant Science ◽  
1995 ◽  
Vol 110 (1) ◽  
pp. 155-164 ◽  
Author(s):  
A. Wilmink ◽  
B.C.E. van de Ven ◽  
J.B.M. Custers ◽  
Y. Nöllen ◽  
J.J.M. Dons
Keyword(s):  
Histological Analysis ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation ◽  
Selective Agents

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

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