scholarly journals Spectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination

2012 ◽  
Vol 54 (4) ◽  
pp. 343-353 ◽  
Author(s):  
K. Schmidt-Rohr ◽  
K. J. Fritzsching ◽  
S. Y. Liao ◽  
Mei Hong
Keyword(s):  
Solid State ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Nmr Spectra ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Two Dimensional ◽  
Spectral Editing ◽  
Protein Resonance Assignment ◽  
Angle Spinning

scholarly journals Dihedral Angle Measurements for Structure Determination by Biomolecular Solid-State NMR Spectroscopy

2021 ◽  
Vol 8 ◽  
Author(s):  
Patrick C. A. van der Wel
Keyword(s):  
Solid State ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Structural Information ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Distance Measurements ◽  
Challenges And Opportunities ◽  
Molecule Interactions ◽  
Angle Spinning

In structural studies of immobilized, aggregated and self-assembled biomolecules, solid-state NMR (ssNMR) spectroscopy can provide valuable high-resolution structural information. Among the structural restraints provided by magic angle spinning (MAS) ssNMR the canonical focus is on inter-atomic distance measurements. In the current review, we examine the utility of ssNMR measurements of angular constraints, as a complement to distance-based structure determination. The focus is on direct measurements of angular restraints via the judicious recoupling of multiple anisotropic ssNMR parameters, such as dipolar couplings and chemical shift anisotropies. Recent applications are highlighted, with a focus on studies of nanocrystalline polypeptides, aggregated peptides and proteins, receptor-substrate interactions, and small molecule interactions with amyloid protein fibrils. The review also examines considerations of when and where ssNMR torsion angle experiments are (most) effective, and discusses challenges and opportunities for future applications.

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