Correlation between cross-bridge kinetics obtained from Trp fluorescence of myofibril suspensions and mechanical studies of single muscle fibers in rabbit psoas

2011 ◽  
Vol 32 (4-5) ◽  
pp. 315-326 ◽  
Author(s):  
Robin Candau ◽  
Masataka Kawai
Keyword(s):  
Muscle Fibers ◽  
Single Muscle ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
Trp Fluorescence ◽  
Mechanical Studies

BDM affects nucleotide binding and force generation steps of the cross-bridge cycle in rabbit psoas muscle fibers

1994 ◽  
Vol 266 (2) ◽  
pp. C437-C447 ◽  
Author(s):  
Y. Zhao ◽  
M. Kawai
Keyword(s):  
Equilibrium Constants ◽  
Muscle Fibers ◽  
Psoas Muscle ◽  
Isometric Tension ◽  
Activation Mechanism ◽  
Phosphate Binding ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
The Cross ◽  
Cross Bridges

The effect of 2,3-butanedione monoxime (BDM) on elementary steps of the cross-bridge cycle was studied with the sinusoidal analysis technique in skinned rabbit psoas muscle fibers. Our results showed that isometric tension and stiffness decreased progressively with an increase in the BDM concentration. The MgATP and MgADP binding constants increased 27 and 6 times, respectively, when BDM was increased from 0 to 18 mM, whereas the phosphate binding constant did not change significantly. The equilibrium constants of the ATP isomerization and detachment step were not sensitive to BDM, whereas the equilibrium constant of the attachment (power stroke) step decreased with BDM. Thus, in the presence of BDM, the number of attached cross bridges decreases; more cross bridges accumulate in the detached state, causing isometric tension and stiffness to decline. However, our detailed analysis shows that the decrease in the number of attached cross bridges is approximately 40%, which is not adequate to account for the 84% decrease in the isometric tension when 18 mM BDM was present. Therefore we suggest that a thin-filament activation mechanism is also affected by BDM.

Cross-bridge movement in glycerinated rabbit psoas muscle fibers

Biochemistry ◽  
1979 ◽  
Vol 18 (6) ◽  
pp. 959-963 ◽  
Author(s):  
Yu-Chih Chen Chiao ◽  
William F. Harrington
Keyword(s):  
Muscle Fibers ◽  
Psoas Muscle ◽  
Rabbit Psoas ◽  
Cross Bridge

scholarly journals Cross-bridge attachment and stiffness during isotonic shortening of intact single muscle fibers

1993 ◽  
Vol 64 (4) ◽  
pp. 1150-1160 ◽  
Author(s):  
P.J. Griffiths ◽  
C.C. Ashley ◽  
M.A. Bagni ◽  
Y. Maéda ◽  
G. Cecchi
Keyword(s):  
Muscle Fibers ◽  
Single Muscle ◽  
Cross Bridge ◽  
Isotonic Shortening
Sign in / Sign up

Export Citation Format

Share Document