A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish

2009 ◽  
Vol 25 (2) ◽  
pp. 339-349 ◽  
Author(s):  
Zhi Wei Wang ◽  
Li Jun Zhang ◽  
Jie Chen ◽  
Chang Ping Xiang ◽  
Shi Yong Mei ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Fertility Restorer ◽  
Intergenic Recombination

A binary vector-based large insert library for Brassica napus and identification of clones linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Yongzhong Wu ◽  
Lomas Tulsieram ◽  
Quangzhou Tao ◽  
Hong-Bin Zhang ◽  
Steven J Rothstein
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Plant Transformation ◽  
Transformation Efficiency ◽  
Binary Vector ◽  
Brassica Species ◽  
Chromosome Walking ◽  
Fertility Restorer ◽  
Plant Transformation Vector

We constructed and characterized a large DNA insert library for Brassica napus that would facilitate genome-related research and map-based cloning efforts in Brassica species. This library, consisting of 92 160 clones arrayed in 384-well microtiter dishes, was based on a conventional plant transformation vector (binary vector), and was constructed using a single ligation with transformation efficiency of over 5000 recombinants per microliter of ligation mixture. Every clone in this library contains an insert in the size range of 30-190 kb, facilitating both chromosome walking and plant transformation. Screening this library with three DNA markers (C2, F10, and CabR) that are linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS) identified at least 17 positive clones for each probe. Among the 17 positive clones identified by C2, nine are linked to the restorer locus. Marker F10 identified 21 clones, of which only two are linked to the restorer locus. None of 68 clones identified by CabR is linked to the restorer locus. A stability test using two clones identified by the C2 marker indicated that large DNA inserts are stable in this conventional vector in both Escherichia coli and Agrobacterium. Key words: Brassica napus, binary vector, large DNA insert library, restorer gene.

Tagging of four fertility restorer loci for wild abortive—cytoplasmic male sterility system in rice (Oryza sativa L.) using microsatellite markers

Euphytica ◽  
2008 ◽  
Vol 164 (3) ◽  
pp. 669-677 ◽  
Author(s):  
L. Bazrkar ◽  
A. J. Ali ◽  
N. A. Babaeian ◽  
A. A. Ebadi ◽  
M. Allahgholipour ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Microsatellite Markers ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Oryza Sativa L ◽  
Fertility Restorer

A new fertility restorer locus linked closely to the Rfo locus for cytoplasmic male sterility in radish

2008 ◽  
Vol 117 (3) ◽  
pp. 313-320 ◽  
Author(s):  
Zhi Wei Wang ◽  
Yu Jie Zhang ◽  
Chang Ping Xiang ◽  
Shi Yong Mei ◽  
Yuan Zhou ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Fertility Restorer

Enarthrocarpus lyratus-based cytoplasmic male sterility and fertility restorer system in Brassica rapa

2003 ◽  
Vol 122 (5) ◽  
pp. 438-440 ◽  
Author(s):  
J. S. Deol ◽  
K. R. Shivanna ◽  
S. Prakash ◽  
S. S. Banga ◽  
G. Robbelen
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Brassica Rapa ◽  
Fertility Restorer

Alloplasmic male sterile Brassica napus with Enarthrocarpus lyratus cytoplasm: introgression and molecular mapping of an E. lyratus chromosome segment carrying a fertility restoring gene

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 792-797 ◽  
Author(s):  
H S Janeja ◽  
S K Banga ◽  
P B Bhaskar ◽  
S S Banga
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Donor Species

A cytoplasmic male sterility (CMS) system for Brassica napus (2n = 38; AACC) was developed by backcross substitution of its nucleus into the cytoplasm of a wild crucifer, Enarthrocarpus lyratus. Male sterility was complete, stable, and expressed in small flowers with rudimentary anthers. Since the B. napus germplasm lines were complete or partial maintainers of male sterility, the required fertility restorer gene (Rfl) was introgressed from the cytoplasm donor species. Inheritance studies carried out on F1 and F2 populations derived from hybridizing cytoplasmic male sterile and male fertile near-isogenic (PNILs) lines of B. napus 'Westar', revealed a monogenic dominant control for fertility restoration. Bulked segregant analysis with 215 RAPD primers helped in the identification of putative primers associated with fertility restoration. Co-segregation analysis of eight such primers with Rfl gene revealed two markers, OPK 15700 and OPZ 061300, which flank the Rfl locus on either side at a distance of 8.2 and 2.5 cM, respectively. These DNA markers will be useful in marker-assisted selection for improving the commercial potential of this newly developed CMS-fertility-restorer system for hybrid seed production programs in rapeseed.Key words: oilseed rape, hybrids, cytoplasmic male sterility, fertility restoration, RAPD mapping.

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