Serological characterization of the new genotype E of small ruminant lentivirus in roccaverano goat flocks

2009 ◽  
Vol 33 (S1) ◽  
pp. 137-140 ◽  
Author(s):  
E. Grego ◽  
D. Lacerenza ◽  
R. Reina Arias ◽  
M. Profiti ◽  
S. Rosati
Keyword(s):  
Small Ruminant ◽  
Serological Characterization ◽  
New Genotype ◽  
Genotype E

Serological characterization of small ruminant lentiviruses: A complete tool for serotyping lentivirus infection in goat

2019 ◽  
Vol 176 ◽  
pp. 42-46 ◽  
Author(s):  
Chiara Nogarol ◽  
Luigi Bertolotti ◽  
Siv Klevar ◽  
Margherita Profiti ◽  
Britt Gjerset ◽  
...  
Keyword(s):  
Small Ruminant ◽  
Serological Characterization ◽  
Small Ruminant Lentiviruses

scholarly journals Genetic characterization of small ruminant lentivirus in Italian mixed flocks: evidence for a novel genotype circulating in a local goat population

2007 ◽  
Vol 88 (12) ◽  
pp. 3423-3427 ◽  
Author(s):  
Elena Grego ◽  
Luigi Bertolotti ◽  
Antonio Quasso ◽  
Margherita Profiti ◽  
Daniela Lacerenza ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Small Ruminant ◽  
Genetic Characterization ◽  
Divergent Sequence ◽  
Serological Identification ◽  
Goat Population ◽  
Novel Genotype ◽  
Genotype A ◽  
Genotype E

In this study, characterization of the gag gene of small ruminant lentiviruses was carried out in Italian mixed flocks. The nearly complete gag gene was amplified and sequenced. Within genotype A, subtype A1 and a novel subtype, A8, were found in goats, and another novel subtype, A9, was found in both sheep and goats. Subtype B1 was found in both host species and subtype B2 was identified only in sheep. A novel, highly divergent sequence was obtained from goats in two epidemiologically related flocks and is proposed to represent a novel genotype, E. Major epitopes of matrix and capsid antigen were highly divergent, suggesting that serological identification of animals infected with genotype E may have been missed by using currently available diagnostic tests. A recombinant subunit ELISA, based on genotype E-specific epitopes, was developed and a third independent flock carrying this genotype was identified, based on serology.

Serological characterization of macrophage hybridomas: Identification of an interferon-γ-inducible surface marker

1988 ◽  
Vol 112 (1) ◽  
pp. 187-199 ◽  
Author(s):  
Carla A. Martin ◽  
Ute Willmer ◽  
Frank W. Falkenberg ◽  
Martin E. Dorf
Keyword(s):  
Interferon Γ ◽  
Surface Marker ◽  
Serological Characterization

Physical, Chemical, and Serological Characterization of Avian Rotaviruses

1988 ◽  
Vol 32 (2) ◽  
pp. 195 ◽  
Author(s):  
S. Y. Kang ◽  
K. V. Nagaraja ◽  
J. A. Newman
Keyword(s):  
Physical Chemical ◽  
Serological Characterization

scholarly journals Purification, Composition, and Serological Characterization of Histoplasmin—H and M Antigens

1974 ◽  
Vol 9 (5) ◽  
pp. 870-880 ◽  
Author(s):  
Georgia Bradley ◽  
Leo Pine ◽  
Michael W. Reeves ◽  
C. Wayne Moss
Keyword(s):  
Serological Characterization

scholarly journals Biological and serological characterization of viruses of summer squash crops in Yugoslavia

2002 ◽  
Vol 47 (2) ◽  
pp. 149-160 ◽  
Author(s):  
Natasa Dukic ◽  
Branka Krstic ◽  
Ivana Vico ◽  
N.I. Katis ◽  
Chryssa Papavassiliou ◽  
...  
Keyword(s):  
Aphis Gossypii ◽  
Virus Transmission ◽  
Leaf Lamina ◽  
Serological Characterization ◽  
Summer Squash ◽  
Leaf Yellowing ◽  
Prevalent Virus ◽  
Viral Nature

A survey on summer squash open field crops was carried out during 2000 and 2001 in order to identify the major viruses infecting these crops in different localities. Plants showed different types of symptoms: mild mosaic, chlorotic spotting, distinctive mosaic, blistering of leaf lamina leaf yellowing, deformation of leaf lamina, knobbed fruits and stunting of plants. The symptoms were very variable but showed the viral nature of the investigated summer squash diseases. The collected samples were tested by bioassay and by two serological methods ELISA and EBIA using cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2), zucchini yellow flack potyvirus (ZYFV) watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) and cucurbit aphid-borne yellows polerovirus (CABYV) polyclonal antisera. In all tested samples single or mixed infection with ZYMV, CMV and WMV-2 was detected. The most prevalent virus infecting summer squash was ZYMV. This is the first report of ZYMV, the most destructive virus infecting cucurbits, in Yugoslavia. It was also proven that the identified viruses are transmissible by Aphis gossypii in a non-persistent manner, but possible role of seed in virus transmission was not confirmed.

Sign in / Sign up

Export Citation Format

Share Document