Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H)

2011 ◽  
Vol 47 (4) ◽  
pp. 516-524 ◽  
Author(s):  
Y. M. Kang ◽  
D. J. Park ◽  
J. Y. Min ◽  
H. J. Song ◽  
M. J. Jeong ◽  
...  
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Methyl Transferase ◽  
Enhanced Production

Enhanced production of tropane alkaloids by nitrate treatment in hairy root cultures of Atropa belladonna

2008 ◽  
Vol 136 ◽  
pp. S55 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Farah Karimi ◽  
Hasan Rahnama
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Atropa Belladonna ◽  
Nitrate Treatment ◽  
Enhanced Production

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Suchitra Banerjee ◽  
L Rahman ◽  
G.C Uniyal ◽  
P.S Ahuja
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production

scholarly journals Production of tropane alkaloids by hairy root cultures of Scopolia japonica.

1986 ◽  
Vol 50 (11) ◽  
pp. 2715-2722 ◽  
Author(s):  
Yoshihiro MANO ◽  
Shigeyasu NABESHIMA ◽  
Chiaki MATSUI ◽  
Hideo OHKAWA
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures

Inducer effect of Tween 20 permeabilization treatment used for release of stored tropane alkaloids inDatura innoxia Mill. hairy root cultures

1996 ◽  
Vol 16 (3-4) ◽  
pp. 241-244
Author(s):  
Michèle Boitel-Conti ◽  
Eric Gontier ◽  
Jean-Claude Laberche ◽  
Corinne Ducrocq ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Tween 20 ◽  
Hairy Root Cultures ◽  
Root Cultures

Incorporation of 1- 13C-Acetate into tropane alkaloids by hairy root cultures of Hyoscyamus albus

1993 ◽  
Vol 32 (4) ◽  
pp. 905-909 ◽  
Author(s):  
Martina Sauerwein ◽  
Koichiro Shimomura ◽  
Michael Wink
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Hyoscyamus Albus
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