Silver nitrate promotes high-frequency multiple shoot regeneration in cotton (Gossypium hirsutum L.) by inhibiting ethylene production and phenolic secretion

2016 ◽  
Vol 52 (4) ◽  
pp. 408-418 ◽  
Author(s):  
G. Prem Kumar ◽  
S. Sivakumar ◽  
G. Siva ◽  
M. Vigneswaran ◽  
T. Senthil Kumar ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Silver Nitrate ◽  
Shoot Regeneration ◽  
Ethylene Production ◽  
High Frequency ◽  
Multiple Shoot ◽  
Gossypium Hirsutum L ◽  
Multiple Shoot Regeneration

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro multiple shoot regeneration from hypocotyl in Menta spicata subsp. spicata

2015 ◽  
Vol 30 (2) ◽  
pp. 126
Author(s):  
Fethi Ahmet Özdemir ◽  
Mehmet Uğur Yıldırım ◽  
Mahsa Pourali Kahriz
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Multiple Shoot Regeneration

Multiple Shoot Regeneration Response of Recalcitrant Cotton (Gossypism hirsutum L.) Cultivar CIM-443

2004 ◽  
Vol 7 (8) ◽  
pp. 1371-1375 ◽  
Author(s):  
Rozina M. Ali ◽  
Tayyab Husnain . ◽  
Syed Sarfraz Hussain . ◽  
Nasir Mahmood . ◽  
Sheikh Riazuddin .
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Regeneration Response ◽  
Multiple Shoot Regeneration
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