Identification and Functional Characterization of a Novel Δ12 Fatty Acid Desaturase Gene from Haematococcus pluvialis

2020 ◽  
Vol 19 (6) ◽  
pp. 1362-1370
Author(s):  
Lin Zhang ◽  
Wenbi Chen ◽  
Shuping Yang ◽  
Yuanbo Zhang ◽  
Jilin Xu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Haematococcus Pluvialis ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene

scholarly journals A novel Delta12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115.

2006 ◽  
Vol 53 (4) ◽  
pp. 753-759 ◽  
Author(s):  
D Sh Wei ◽  
M Ch Li ◽  
X X Zhang ◽  
H Zhou ◽  
L J Xing
Keyword(s):  
Fatty Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Methylotrophic Yeast ◽  
Pastoris Gs115 ◽  
Desaturase Gene ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Membrane Bound ◽  
Fatty Acid Desaturase Gene

The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Delta12 and Delta15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Delta12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Delta12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Delta15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Delta12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Delta12-fatty acid desaturase gene in methylotrophic yeast.

Identification and Functional Characterization of the Delta 6-Fatty Acid Desaturase Gene from Thamnidium elegans

2007 ◽  
Vol 54 (1) ◽  
pp. 110-117 ◽  
Author(s):  
DEPEI WANG ◽  
MINGCHUN LI ◽  
DONGSHENG WEI ◽  
YI CAI ◽  
YINGHUI ZHANG ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene

Characterization of a Δ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus

2010 ◽  
Vol 87 (1) ◽  
pp. 215-224 ◽  
Author(s):  
Takahito Watanabe ◽  
Saeko Tsuda ◽  
Hiroshi Nishimura ◽  
Yoichi Honda ◽  
Takashi Watanabe
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturase ◽  
Desaturase Gene ◽  
Ceriporiopsis Subvermispora ◽  
Fatty Acid Desaturase Gene

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

Identification and characterization of a novel Δ12-fatty acid desaturase gene fromRhizopus arrhizus

FEBS Letters ◽  
2004 ◽  
Vol 573 (1-3) ◽  
pp. 45-50 ◽  
Author(s):  
Dongsheng Wei ◽  
Mingchun Li ◽  
Xinxin Zhang ◽  
Yong Ren ◽  
Laijun Xing
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturase ◽  
Desaturase Gene ◽  
Identification And Characterization ◽  
Fatty Acid Desaturase Gene
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