Aptamer-Based DNA Materials for the Separation and Analysis of Biological Particles

Author(s):  
Chenxu Zhu ◽  
Chi Yao ◽  
Dayong Yang
2002 ◽  
Vol 2 ◽  
pp. 1440-1443 ◽  
Author(s):  
John Graham

Any density gradient for the isolation of mammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep™ in order to prepare such solutions for mammalian organelles and membranes.


2004 ◽  
Author(s):  
Matthew B. Hart ◽  
Horn-Bond Lin ◽  
Jason Deich ◽  
Alan L. Huston ◽  
Jay D. Eversole ◽  
...  

Author(s):  
Olga Kononova ◽  
Kenneth A. Marx ◽  
Valeri Barsegov
Keyword(s):  

Author(s):  
Steven C. Hill ◽  
Ronald G. Pinnick ◽  
Stanley Niles ◽  
Yong-Le Pan ◽  
Stephen Holler ◽  
...  

2015 ◽  
Vol 15 (12) ◽  
pp. 16273-16323 ◽  
Author(s):  
R. H. Mason ◽  
M. Si ◽  
J. Li ◽  
C. Chou ◽  
R. Dickie ◽  
...  

Abstract. Information on what aerosol particle types are the major sources of ice nucleating particles (INPs) in the atmosphere is needed for climate predictions. To determine which aerosol particles are the major sources of immersion-mode INPs at a coastal site in Western Canada, we investigated correlations between INP number concentrations and both concentrations of different atmospheric particles and meteorological conditions. We show that INP number concentrations are strongly correlated with the number concentrations of fluorescent bioparticles between −15 and −25 °C, and that the size distribution of INPs is most consistent with the size distribution of fluorescent bioparticles. We conclude that biological particles were likely the major source of ice nuclei at freezing temperatures between −15 and −25 °C at this site for the time period studied. At −30 °C, INP number concentrations are also well correlated with number concentrations of the total aerosol particles ≥ 0.5 μm, suggesting that non-biological particles may have an important contribution to the population of INPs active at this temperature. As we found that black carbon particles were unlikely to be a major source of ice nuclei during this study, these non-biological INPs may include mineral dust. Furthermore, correlations involving tracers of marine aerosols and marine biological activity indicate that the majority of INPs measured at the coastal site likely originated from terrestrial rather than marine sources. Finally, six existing empirical parameterizations of ice nucleation were tested to determine if they accurately predict the measured INP number concentrations. We found that none of the parameterizations selected are capable of predicting INP number concentrations with high accuracy over the entire temperature range investigated.


Sign in / Sign up

Export Citation Format

Share Document