The effects of plant growth regulators, activated charcoal, and AgNO3 on microspore derived embryo formation in broccoli (Brassica oleracea L. var. italica)

2011 ◽  
Vol 52 (5) ◽  
pp. 524-529 ◽  
Author(s):  
Haeyoung Na ◽  
Jung-Ho Kwak ◽  
Changhoo Chun
2011 ◽  
Vol 74 (3) ◽  
pp. 193-198 ◽  
Author(s):  
Ewa Łojkowska ◽  
Aleksandra Królicka ◽  
Matylda Sidwa-Gorycka ◽  
Jan J. Rybczyński ◽  
Dariusz L. Szlachetko ◽  
...  

In order to estimate the best germination conditions of <em>Encyclia</em> aff. <em>oncidioides</em> seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went) with different concentrations of plant growth regulators such as benzyladenine (BA), naphthaleneacetic acid (NAA) and gibberellic acid (GA3) were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA<sub>3</sub> inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB<sub>2</sub> media with 0.2% activated charcoal proved to be the best for <em>E.</em> aff. <em>oncidioides</em> seed germination, seedling development and plantlet propagation.


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