The aim of this study was to determine the possibility of micropropagation of
the medicinal plant A. millefolium on half-strength MS medium and ex vitro
rooting and acclimatization of the obtained microshoots in hydroculture in
order to establish an efficient production method. Two explant types were
used: basal and terminal cuttings, and better results were achieved when
terminal cuttings were used. The development of shoots in the multiplication
phase was successful with a regeneration percentage of 100%. Ex vitro rooting
in a modified Hoagland nutrient solution was successful (83%), but the
percentage of in vitro rooting on half-strength MS medium without hormones
was higher (95%). However, bearing in mind that mass production of A.
millefolium is more efficient when the phase of in vitro rooting is excluded,
this method could be recommended for commercial propagation of this medicinal
plant. It is necessary to conduct additional research in order to optimize
the composition, EC and pH value of the hydroponic nutrient solution.
New insights for the production of medicinal plant materials: ex vitro and in vitro propagation of valuable Lamiaceae species from northern Africa
