Mutant Isolation

Keyword(s):  

Author(s):  
Pierre Thuriaux ◽  
Carl Mann ◽  
Jean-Marie Buhler ◽  
Isabelle Treich ◽  
Rosmarie Gudenus ◽  
...  


Genetics ◽  
1988 ◽  
Vol 118 (4) ◽  
pp. 627-636
Author(s):  
E P Hamilton ◽  
P B Suhr-Jessen ◽  
E Orias

Abstract Vinblastine is shown to induce pronuclear fusion failure in conjugating Tetrahymena thermophila. In this alternate conjugational pathway gametic pronuclei are exchanged between conjugants but do not fuse. Each pronucleus undergoes one mitotic division to produce a new macro- and micronucleus. Genetic consequences of pronuclear fusion failure include the following: (1) the progeny are whole genome homozygotes with nuclei derived from single meiotic products, and (2) half of the progeny are heterokaryons with micro- and macronuclei of different genetic origins. These facts make this process extremely useful in strain construction and mutant isolation. The induction of pronuclear fusion failure by vinblastine suggests that microtubules play an essential role in pronuclear fusion.



1993 ◽  
Vol 75 (5) ◽  
pp. 359-363 ◽  
Author(s):  
Katsuhiko Kitamoto ◽  
Kaoko Oda-Miyazaki ◽  
Katsuya Gomi ◽  
Chieko Kumagai


1992 ◽  
Vol 56 (3) ◽  
pp. 417-420
Author(s):  
Benjamin A. Horwitz ◽  
Rivka Amit ◽  
Edward D. Llpson
Keyword(s):  


1999 ◽  
Vol 172 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Young-eun Leem ◽  
Soon-ja Kim ◽  
Ian K. Ross ◽  
Hyoung T. Choi


1967 ◽  
Vol 13 (2) ◽  
pp. 205-215 ◽  
Author(s):  
YAO-TUNG LIU ◽  
HAJIME TAKAHASHI ◽  
BUNJI MARUO




Author(s):  
Larry H. Thompson
Keyword(s):  


Sign in / Sign up

Export Citation Format

Share Document