Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12

1977 ◽  
Vol 113 (1) ◽  
pp. 1-25 ◽  
Author(s):  
Haruo Saito ◽  
Hisao Uchida
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Bacteriophage Lambda ◽  
Escherichia Coli K12

scholarly journals Inhibition of transcription starting from bacteriophage lambda pR promoter during the stringent response in Escherichia coli: implications for lambda DNA replication.

1995 ◽  
Vol 42 (2) ◽  
pp. 233-239 ◽  
Author(s):  
A Szalewska-Pałasz ◽  
G Wegrzyn
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Dna Replication ◽  
Transcriptional Activation ◽  
Plasmid Dna ◽  
Bacteriophage Lambda ◽  
Stringent Response ◽  
Lacz Gene ◽  
Relative Level ◽  
Lambda Dna

Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fusion which consists of the lacZ gene under the control of bacteriophage lambda pR promoter we found that transcription starting from this promoter was inhibited during the stringent, but not the relaxed, response in E. coli. We confirmed our conclusion by estimating the relative level of the pR transcript by RNA-DNA hybridization. We propose that decreased transcription from the pR promoter which serves as transcriptional activation of ori lambda is responsible for inhibition of lambda plasmid replication during the stringent response. The results presented in this paper, combined with our recent findings (published elsewhere), indicate that the transcriptional activation of ori lambda may be a main regulatory process controlling lambda DNA replication not only during the relaxed response but also in normal growth conditions.

A DNA replication gene maps near terC in Escherichia coli K12

1991 ◽  
Vol 226-226 (1-2) ◽  
pp. 315-317 ◽  
Author(s):  
Talia Ben-Neria ◽  
Eliora Z. Ron
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Escherichia Coli K12 ◽  
Replication Gene ◽  
Gene Maps
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