In vitro effects of progesterone and estradiol-17β on choleragen activated Xenopus oocyte adenylate cyclase

1982 ◽  
Vol 17 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Catherine Thibier ◽  
Odile Mulner ◽  
René Ozon
Keyword(s):  
Adenylate Cyclase ◽  
Xenopus Oocyte ◽  
In Vitro Effects ◽  
Estradiol 17Β

Adrenal adenylate cyclase and steroidogenic activities of 63 day old ovine fetuses: in vitro effects of ACTH1−24 and forskolin

Biochimie ◽  
1987 ◽  
Vol 69 (6-7) ◽  
pp. 629-638 ◽  
Author(s):  
Philippe Durand ◽  
Anne-Marie Cathiard ◽  
Elisabeth Naaman ◽  
Saez JoséM
Keyword(s):  
Adenylate Cyclase ◽  
In Vitro Effects

Short-term effect of aldosterone on vasopressin-sensitive adenylate cyclase in rat collecting tubule

1993 ◽  
Vol 264 (5) ◽  
pp. F821-F826 ◽  
Author(s):  
G. el Mernissi ◽  
C. Barlet-Bas ◽  
C. Khadouri ◽  
L. Cheval ◽  
S. Marsy ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Affinity Constant ◽  
Short Term ◽  
Collecting Tubule ◽  
Lag Period ◽  
In Vitro Effects ◽  
Collecting Tubules ◽  
Adrenalectomized Rats

Because previous studies indicated that, in the rat collecting tubule, vasopressin (AVP)-sensitive adenylate cyclase (AC) is controlled by mineralocorticoids in the long term, the present study was designed to investigate whether such a control also exists in the short term. Therefore, we investigated the in vivo and in vitro effects of aldosterone on AC activity in cortical and outer medullary collecting tubules (CCD and OMCD, respectively) from adrenalectomized rats. Injection of aldosterone (10 micrograms/kg body wt) to adrenalectomized rats restored within 3 h AVP-sensitive AC activity in the CCD and OMCD up to the levels observed in the corresponding segments of adrenal intact rats. Similarly, incubating CCD or OMCD from adrenalectomized rats for 2.5 h in the presence of 10(-8) M aldosterone enhanced AVP-sensitive AC activity up to values similar to those found in normal rats. In vitro stimulation of AVP-sensitive AC activity was dose dependent with regard to aldosterone [apparent affinity constant (K0.5) approximately 10(-9) M], appeared after a 30-min lag period, and reached its maximum after 2-2.5 h. In addition, it was totally abolished by the antimineralocorticoid spironolactone, whereas the specific glucocorticoid antagonist RU 38486 had no effect. Finally, actinomycin D and cycloheximide totally abolished the in vitro action of aldosterone, demonstrating the involvement of protein synthesis in that process.

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