The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus)

The in vitro effects of protease inhibitors on chorion expansion and the fertility of goldfish (Carassius auratus) eggs were investigated. Expansion of the chorion in ovulated eggs was blocked by the serine protease inhibitors benzamidine and soybean trypsin inhibitor when they were held in a goldfish Ringer solution. Eggs in which chorion expansion was inhibited retained their fertility for 30 min when held in this solution. In contrast, ovulated eggs lost their fertility in freshwater even in the presence of a protease inhibitor. The results suggest that chorion expansion and loss of fertility in water-activated goldfish eggs may involve proteolysis which was blocked by benzamidine and soybean trypsin inhibitor. Moreover, the blockage of chorion expansion may be pivotal to the preservation of fertility in eggs held in Ringer's solution with soybean trypsin inhibitor. The development of this solution provides a means to temporarily hold ovulated goldfish eggs in a viable state outside the female and will permit further studies on fertilization in this species.

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In vitro effects of thyroid hormones on gonadotropin-induced estradiol-17β secretion by ovarian follicles of rainbow trout, Salmo gairdneri

General and Comparative Endocrinology ◽

10.1016/0016-6480(88)90055-x ◽

1988 ◽

Vol 69 (1) ◽

pp. 80-87 ◽

Cited By ~ 41

Author(s):

Daniel G. Cyr ◽

J.G. Eales

Keyword(s):

Rainbow Trout ◽

Thyroid Hormones ◽

Ovarian Follicles ◽

Salmo Gairdneri ◽

In Vitro Effects ◽

Estradiol 17Β

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Oxoanions stimulate in vitro ovulation and signal transduction pathways in goldfish (Carassius auratus) follicles

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1992.263.5.e943 ◽

1992 ◽

Vol 263 (5) ◽

pp. E943-E949 ◽

Cited By ~ 1

Author(s):

S. Y. Hsu ◽

F. W. Goetz

Keyword(s):

Signal Transduction ◽

Carassius Auratus ◽

Sodium Tungstate ◽

Sodium Molybdate ◽

Vanadyl Sulfate ◽

Signal Transduction Pathways ◽

Sodium Selenate ◽

Sodium Chromate ◽

Goldfish Carassius Auratus

The present study investigated the effects of a number of oxoanion compounds on in vitro ovulation of goldfish follicles and ovarian second messenger activities. Significant levels of ovulation were induced by 0.1 mM sodium chromate, 0.1 mM sodium metavanadate, 10 mM sodium molybdate, 0.1 mM sodium orthovanadate, 5 mM sodium selenate, 0.5 mM sodium tungstate, and 0.1 mM vanadyl sulfate. At levels that significantly stimulated ovulation, metavanadate, molybdate, orthovanadate, tungstate, and vanadyl sulfate also stimulated follicular phosphatidylinositol cycling and inhibited ovarian alkaline phosphatase activity. Moreover, the ovulation induced by these oxoanions was not inhibited by indomethacin (10 micrograms/ml), while ovulation induced by selenate and chromate was. In contrast, only vanadium-containing compounds significantly stimulated prostaglandin (PG) synthesis, and, in fact, selenate significantly inhibited PG production. Finally, only sodium molybdate- and vanadium-containing compounds appeared to increase follicular adenosine 3',5'-cyclic monophosphate content. While all oxoanions stimulated in vitro ovulation, they had differential effects on certain signal transduction pathways when tested at concentrations that stimulated in vitro ovulation. From the results, two basic groups could be delineated, one containing tungstate-, molybdate-, and vanadium-containing compounds and the other selenate and chromate. Thus the mechanism by which ovulation is induced by chromate and selenate may be different from that of vanadium-containing compounds, molybdate, and tungstate.(ABSTRACT TRUNCATED AT 250 WORDS)

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