Direct comparison, in human resting lymphocytes, of the inter-individual variations in unscheduled DNA synthesis induced by N-acetoxy-2-acetylaminofluorene and ultraviolet irradiation

1980 ◽  
Vol 73 (2) ◽  
pp. 349-361 ◽  
Author(s):  
Ronald W. Pero ◽  
Catharina Östlund
Keyword(s):  
Dna Synthesis ◽  
Ultraviolet Irradiation ◽  
Unscheduled Dna Synthesis ◽  
Individual Variations ◽  
Resting Lymphocytes

scholarly journals Lesions induced in DNA by ultraviolet light are repaired at the nuclear cage

1984 ◽  
Vol 70 (1) ◽  
pp. 189-196
Author(s):  
S.J. McCready ◽  
P.R. Cook
Keyword(s):  
Dna Synthesis ◽  
Ultraviolet Light ◽  
Hela Cells ◽  
Nuclear Matrix ◽  
Ultraviolet Irradiation ◽  
Mammalian Cells ◽  
Nuclear Dna ◽  
S Phase ◽  
Unscheduled Dna Synthesis ◽  
Phase Synthesis

In mammalian cells, S-phase DNA synthesis occurs at sites fixed to a sub-nuclear structure, the nuclear matrix or cage. This is an ordered network of non-histone proteins, which maintains its essential morphology even in the absence of DNA. We show here that unscheduled DNA synthesis following exposure of HeLa cells to ultraviolet light also takes place at this sub-structure. We also show that ultraviolet irradiation grossly reorganizes nuclear DNA, arresting S-phase synthesis at the cage and leaving the residual synthesis highly localized.

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