Nuclear matrix associated RNA datasets of posterior silk glands of Bombyx mori during 5th instar larval development

Objectives Bombyx mori is the key contributor to industrial silk production. The maximum production of silk occurs during 5th instar. The posterior silk glands in the larvae are responsible for the production of the main component of silk fibre—fibroin. The expression of genes and their regulation are dependent on the chromatin architecture. The nuclear matrix supports its structure and function by anchoring specific regions to regulate gene expression. The major constituent of the nuclear matrix, crucial to its structural and temporal maintenance, is its RNA. Therefore, the study of nuclear matrix RNA of the posterior silk glands on different days of 5th instar larval development is essential to understand its association to differential expression of genes. Data description The tissue-specific developmental association of nuclear matrix RNA (NuMat RNA) at the genome level has not been done so far for any organism. Bombyx mori, CSR2 X CSR4 is the most popular dihybrid strain in India. The nuclear matrix RNA was isolated from day 1, day 5 and day 7 of 5th instar posterior silk glands of Bombyx mori. The NuMat RNA was sequenced using Illumina platform. The reads obtained were processed and the datasets were deposited in NCBI.

Interacting Shell Model Calculations for Neutrinoless Double Beta Decay of 82Se With Left-Right Weak Boson Exchange

In the present work, the λ mechanism (left-right weak boson exchange) and the light neutrino-exchange mechanism of neutrinoless double beta decay is studied. In particular, much attention is paid to the calculation of nuclear matrix elements for one of the neutrinoless double beta decaying isotopes 82Se. The interacting shell model framework is used to calculate the nuclear matrix element. The widely used closure approximation is adopted. The higher-order effect of the pseudoscalar term of nucleon current is also included in some of the nuclear matrix elements that result in larger Gamow-Teller matrix elements for the λ mechanism. Bounds on Majorana neutrino mass and lepton number violating parameters are also derived using the calculated nuclear matrix elements.

In situ Nuclear Matrix preparation in Drosophila melanogaster and its use in studying the components of nuclear architecture

The study of Nuclear Matrix (NuMat) over the last 40 years has been limited to either isolated nuclei from tissues or cells grown in culture. Here, we provide a protocol for NuMat preparation in intact Drosophila melanogaster embryos and its use in dissecting the components of nuclear architecture. The protocol does not require isolation of nuclei and therefore maintains the three-dimensional milieu of an intact embryo, which is biologically more relevant compared to cells in culture. One of the advantages of this protocol is that only a small number of embryos are required. The protocol can be extended to larval tissues like salivary glands and imaginal discs with little modification. Taken together, it becomes possible to carry out such studies in parallel to genetic experiments using mutant and transgenic flies. This protocol, therefore, opens the powerful field of fly genetics to cell biology in the study of nuclear architecture.

Diagnostic accuracy of NMP-52, NMP-22 and urine cytology in the diagnosis of bladder cancer

Bladder cancer (BC) is the most important tumor problem of urologic cancer. Therefore, noninvasive urinary biomarkers were used for diagnosis of BC. However, the new biomarkers failed to reach higher accuracy. The aim of this study was to assess the diagnostic efficacy of nuclear matrix protein-22 (NMP- 22), nuclear matrix protein-52 (NMP-52), urinary cytology and to investigate combinations of urine NMP-52 with urinary cytology as noninvasive biomarkers to increase diagnostic performance of bladder cancer at different grades and stages. Overall, there were 156 subjects (62 BC, 54 cystitis patients and 40 healthy volunteers). The NMP-22 and NMP-52 were quantified in urine samples by ELISA. The urinary cytology is used by some physicians routinely for diagnosis of BC. The sensitivity and specificity for NMP-52 were 94% and 82%, for NMP-22 69% and 80.8% and for cytology 56% and 94.6% respectively and also, both urinary NMP-22 and NMP-52 have extremely significant relation (p<0.0001) to BC vs. healthy individuals and cystitis patients. Moreover, the combination of NMP- 52 with urinary cytology could predict all BC stages and grade with 95.6% sensitivity and 94.3% specificity. In conclusion, NMP-52 and urinary cytology in combination improve diagnostic performance for BC detection in different pathological types.

Estimation of nuclear matrix elements of double-$$\varvec{\beta }$$ decay from shell model and quasiparticle random-phase approximation

The nuclear matrix element (NME) of neutrinoless double-$$\beta $$ β ($$0\nu \beta \beta $$ 0 ν β β ) decay is an essential input for determining the neutrino effective mass, if the half-life of this decay is measured. Reliable calculation of this NME has been a long-standing problem because of the diversity of the predicted values of the NME, which depends on the calculation method. In this study, we focus on the shell model and the QRPA. The shell model has a rich amount of the many-particle many-hole correlations, and the quasiparticle random-phase approximation (QRPA) can obtain the convergence of the calculation results with respect to the extension of the single-particle space. It is difficult for the shell model to obtain the convergence of the $$0\nu \beta \beta $$ 0 ν β β NME with respect to the valence single-particle space. The many-body correlations of the QRPA may be insufficient, depending on the nuclei. We propose a new method to phenomenologically modify the results of the shell model and the QRPA compensating for the insufficiencies of each method using the information of other methods in a complementary manner. Extrapolations of the components of the $$0\nu \beta \beta $$ 0 ν β β NME of the shell model are made toward a very large valence single-particle space. We introduce a modification factor to the components of the $$0\nu \beta \beta $$ 0 ν β β NME of the QRPA. Our modification method yields similar values of the $$0\nu \beta \beta $$ 0 ν β β NME for the two methods with respect to $$^{48}$$ 48 Ca. The NME of the two-neutrino double-$$\beta $$ β decay is also modified in a similar but simpler manner, and the consistency of the two methods is improved.

ROJAS-BERMÚDEZ’S NUCLEUS OF THE EGO THEORY AND ITS CORRELATION WITH IMAGODRAMA

The purpose of this article is to present a theoretical reading of the nucleus of the ego, closer to the theory of the identity matrix by J. L. Moreno, deeply developing the Rojas-Bermúdez’s concept of incipient ego. I associate this egoic experience with the relationships established between the baby and its family nucleus, right after recognizing a minimally stable ego, in neuropsychological conditions to link itself to the maternal, paternal and fraternal images. According to this reading, the nucleus of the ego completes its structuring at the age of four, following the Rojas-Bermúdez School, after carrying out the process of synthesis of this nuclear matrix, from which the natural ego emerges and, later, the social ego, as a product of the triangulation and circularization of roles. The image construction technique is the method that supports the theory in question.