A comparison of electrophoretic methods for isoenzyme characterization of trypanosomatids. I: Standard stocks of Trypanosoma cruzi zymodemes from northeast Brazil

1981 ◽  
Vol 75 (5) ◽  
pp. 742-750 ◽  
Author(s):  
Sheila M. Lanham ◽  
Judy M. Grendon ◽  
M.A. Miles ◽  
Marinette M. Povoa ◽  
A.A.Almeida de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Northeast Brazil

scholarly journals Characterization of a Trypanosoma cruzi strain isolated from a non-endemic area in Northeast Brazil

1995 ◽  
Vol 37 (1) ◽  
pp. 87-89 ◽  
Author(s):  
Yara de Miranda Gomes ◽  
Tereza Cristina A. Leal ◽  
Marlene R. Silva ◽  
Christiane M. G. Santiago ◽  
Eridan M. Coutinho
Keyword(s):  
Trypanosoma Cruzi ◽  
Endemic Area ◽  
Northeast Brazil

In silico integrative analysis for the characterization of LYT1 a unique protein of Trypanosoma cruzi

2021 ◽  
pp. 1-7
Author(s):  
María Virginia Ramirez-Montoya ◽  
Danielle García-Olivares ◽  
Héctor Acosta ◽  
Ascanio Rojas
Keyword(s):  
Trypanosoma Cruzi ◽  
In Silico ◽  
Integrative Analysis ◽  
Unique Protein

scholarly journals Molecular and biological characterization of Trypanosoma cruzi strains isolated from children from Jequitinhonha Valley, State of Minas Gerais, Brazil

2013 ◽  
Vol 46 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Jaquelline Carla Valamiel de Oliveira e Silva ◽  
Girley Francisco Machado de Assis ◽  
Maykon Tavares de Oliveira ◽  
Helder Magno Silva Valadares ◽  
Italo Faria do Valle ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Minas Gerais ◽  
Biological Characterization

In silico structural characterization of protein targets for drug development against Trypanosoma cruzi

2016 ◽  
Vol 22 (10) ◽  
Author(s):  
Carlyle Ribeiro Lima ◽  
Nicolas Carels ◽  
Ana Carolina Ramos Guimaraes ◽  
Pierre Tufféry ◽  
Philippe Derreumaux
Keyword(s):  
Trypanosoma Cruzi ◽  
Drug Development ◽  
Structural Characterization ◽  
In Silico ◽  
Protein Targets

DNA fingerprinting of Trypanosoma cruzi: A new tool for characterization of strains and clones

1992 ◽  
Vol 55 (1-2) ◽  
pp. 147-153 ◽  
Author(s):  
Andrea M. Macedo ◽  
Maria Sonia Martins ◽  
Egler Chiari ◽  
Sergio D.J. Pena
Keyword(s):  
Trypanosoma Cruzi ◽  
Dna Fingerprinting

scholarly journals Characterization of a Gi-protein from Trypanosoma cruzi epimastigote membranes

1992 ◽  
Vol 287 (2) ◽  
pp. 443-446 ◽  
Author(s):  
O A Coso ◽  
A Díaz Añel ◽  
H Martinetto ◽  
J P Muschietti ◽  
M Kazanietz ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Pertussis Toxin ◽  
Polyclonal Antibodies ◽  
Gel Filtration ◽  
Binding Activity ◽  
Gi Protein ◽  
Liver Membranes ◽  
Blocking Capacity ◽  
Polypeptide Band

A guanosine 5′-[gamma-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this blocking capacity. Incubation of T. cruzi membranes with activated pertussis toxin and [adenylate-32P]NAD+ led to the incorporation of radioactivity into a labelled product with an apparent M(r) of approx. 43,000. Crude membranes were electrophoresed on SDS/polyacrylamide gels and analysed, by Western blotting, with GA/1 anti-alpha common, AS/7 anti-alpha t, anti-alpha i1 and anti-alpha i2 polyclonal antibodies. These procedures led to the identification of a specific polypeptide band of about 43 kDa. Another polypeptide reacting with the SW/1 anti-beta antibody, of about 30 kDa, was also detected in the membrane fraction.

Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA

1992 ◽  
Vol 86 (3) ◽  
pp. 231-237 ◽  
Author(s):  
T. Mimori ◽  
M. Maldonado ◽  
M. Samudio ◽  
A. Rojas De Arias ◽  
R. Moreno ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Kinetoplast Dna

Trypanosoma cruzi: Isolation of Cloned Strains and Characterization of Their Infectivity

1985 ◽  
Vol 71 (4) ◽  
pp. 397 ◽  
Author(s):  
Amilcar Tanuri ◽  
Paulo P. de Andrade ◽  
Darcy F. de Almeida
Keyword(s):  
Trypanosoma Cruzi
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