Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA

1992 ◽  
Vol 86 (3) ◽  
pp. 231-237 ◽  
Author(s):  
T. Mimori ◽  
M. Maldonado ◽  
M. Samudio ◽  
A. Rojas De Arias ◽  
R. Moreno ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Kinetoplast Dna

Characterization of the DNA of Canine Adenovirus by Restriction Enzyme Analysis

Intervirology ◽  
1985 ◽  
Vol 23 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Gholamreza Darai ◽  
Angela Rösen ◽  
Hajo Delius ◽  
Rolf M. Flügel
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

scholarly journals Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies

1989 ◽  
Vol 12 (2) ◽  
pp. 139-157 ◽  
Author(s):  
Harrie G.A.M. van der Avoort ◽  
Anton G. Wermenbol ◽  
Timo P.L. Zomerdijk ◽  
John A.F.W. Kleijne ◽  
Jack A.A.M. van Asten ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Dna Restriction

scholarly journals Molecular Epidemiological Characterization of Respiratory Isolates ofMoraxella catarrhalisin a Pediatric Intensive Care Unit

1992 ◽  
Vol 3 (4) ◽  
pp. 189-192 ◽  
Author(s):  
Anne G Matlow ◽  
Donald E Low ◽  
Gideon Paret ◽  
Scott Jarrett ◽  
Desmond Bohn ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Pediatric Intensive Care Unit ◽  
Restriction Enzyme ◽  
Pediatric Intensive Care ◽  
Respiratory Pathogen ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Strain Isolation

A perceived increase in the number of isolates ofMoraxella catarrhalisfrom the respiratory secretions of patients intubated in the pediatric intensive care unit prompted a review of the clinical profiles of such patients and restriction enzyme analysis of the strains involved. Over two months, of 192 patients admitted to the unit, 154 were intubated. Of the 46 for whom endotracheal tube specimens were submitted to the laboratory,M catarrhaliswas isolated in 12.M catarrhaliswas not felt to be a significant respiratory pathogen by the attending medical staff in any of the patients from whom it was isolated. In only two patients (17%) could nosocomial acquisition be firmly invoked. Restriction enzyme analysis of the 12 strains ruled out the presence of an epidemic strain. Isolation ofM catarrhalisfrom intubated children does not necessarily imply pathogenicity nor an outbreak situation.

Study on genetic polymorphism of Leishmania infantum through the analysis of restriction enzyme digestion patterns of kinetoplast DNA

Parasitology ◽  
1989 ◽  
Vol 99 (3) ◽  
pp. 301-309 ◽  
Author(s):  
M. C. Angelici ◽  
M. Gramiccia ◽  
L. Gradoni
Keyword(s):  
Restriction Enzyme ◽  
Leishmania Infantum ◽  
Enzyme Digestion ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Restriction Enzyme Digestion ◽  
Kinetoplast Dna ◽  
Black Rat ◽  
Electrophoretic Patterns ◽  
Human Cutaneous Leishmaniasis

SummaryTwenty-nine Leishmania infantum strains characterized by different host source, tropism and belonging to 6 zymodemes, were examined by restriction enzyme analysis of kinetoplast DNA (kDNA) using 15 endonucleases. The enzymes which produced only one fragment revealed full identity between all the strains examined, while those producing many bands gave different electrophoretic patterns. They were interpreted with the aid of numerical analyses (cluster and multifactorial analysis). The results show a cline of genetic variability among the strains, the highest similarity being observed between most of the viscerotropic strains isolated from man, dog, black rat and sandflies. The strain agents of human cutaneous leishmaniasis show a varying degree of genetic divergence from this group, which appears more evident when characters from isoenzymes are considered.

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