An in vitro focus-induction assay for xenotropic murine leukemia virus, feline leukemia virus C, and the feline-primate viruses RD-114/CCC/M-7

Virology ◽  
1975 ◽  
Vol 67 (1) ◽  
pp. 288-291 ◽  
Author(s):  
Paul T. Peebles
Retrovirology ◽  
2011 ◽  
Vol 8 (Suppl 1) ◽  
pp. A236
Author(s):  
Romana Hadravová ◽  
Jitka Štokrová ◽  
Michal Doležal ◽  
Iva Pichová ◽  
Tomáš Ruml ◽  
...  

1983 ◽  
Vol 45 (1) ◽  
pp. 473-477 ◽  
Author(s):  
M D Hoggan ◽  
C E Buckler ◽  
J F Sears ◽  
W P Rowe ◽  
M A Martin

2017 ◽  
Vol 114 (10) ◽  
pp. 2723-2728 ◽  
Author(s):  
Mathilda Sjöberg ◽  
Robin Löving ◽  
Birgitta Lindqvist ◽  
Henrik Garoff

Viral membrane fusion proteins of class I are trimers in which the protomeric unit is a complex of a surface subunit (SU) and a fusion active transmembrane subunit (TM). Here we have studied how the protomeric units of Moloney murine leukemia virus envelope protein (Env) are activated in relation to each other, sequentially or simultaneously. We followed the isomerization of the SU-TM disulfide and subsequent SU release from Env with biochemical methods and found that this early activation step occurred sequentially in the three protomers, generating two asymmetric oligomer intermediates according to the scheme (SU-TM)3→ (SU-TM)2TM → (SU-TM)TM2→ TM3. This was the case both when activation was triggered in vitro by depleting stabilizing Ca2+from solubilized Env and when viral Env was receptor triggered on rat XC cells. In the latter case, the activation reaction was too fast for direct observation of the intermediates, but they could be caught by alkylation of the isomerization active thiol.


2014 ◽  
Vol 47 (3) ◽  
pp. 302-306 ◽  
Author(s):  
Samara Tatielle Monteiro Gomes ◽  
Luciana Imbiriba ◽  
Rommel Rodriguéz Burbano ◽  
Artur Luiz da Costa Silva ◽  
Rosimar Neris Martins Feitosa ◽  
...  

Science ◽  
1971 ◽  
Vol 172 (3990) ◽  
pp. 1353-1355 ◽  
Author(s):  
L. D. Gelb ◽  
S. A. Aaronson ◽  
M. A. Martin

Retrovirology ◽  
2010 ◽  
Vol 7 (1) ◽  
pp. 113 ◽  
Author(s):  
Jonathan P Stoye ◽  
Robert H Silverman ◽  
Charles A Boucher ◽  
Stuart FJ Le Grice

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