90 Detection of chromosomal aberrations in interphase nuclei from human primary breast tumors using in situ hybridization with chromosome-specific repetitive DNA-probes

The 3HS.3BL spontaneous Robertsonian translocation obtained from the progenies of wheat–barley (Chinese Spring × Betzes) hybrids backcrossed with wheat line Mv9kr1 was transferred into the modern Martonvásár wheat cultivar Mv Bodri. The translocation was identified with molecular cytogenetic methods. The inheritance of the translocation was traced using genomic in situ hybridization. Fluorescence in situ hybridization using barley subtelomeric (HvT01) and centromere-specific [(AGGGAG)4] repetitive DNA probes confirmed that the complete barley chromosome arm was involved in the Robertsonian translocation. The wheat-specific repetitive DNA probes identified the presence of the whole wheat genome, except the short arm of the 3B chromosome. Genotypes homozygous for the centric fusion were selected, after which morphological analysis was performed on the plants and the yield components were measured in the field during two consecutive vegetative seasons. The introgression of the 3HS.3BL translocation into the modern wheat cultivar Mv Bodri significantly reduced the plant height due to the incorporation of the dwarfing allele RhtD1b. The presence of the 3HS.3BL translocation in the Mv9kr1 and Mv Bodri wheat background improved tillering and seeds per plant productivity in field experiments carried out in Martonvásár and Keszthely, Hungary.

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Rapid fluorescence in situ hybridization with repetitive DNA probes: Quantification by digital image analysis

Cytometry ◽

10.1002/cyto.990170103 ◽

1994 ◽

Vol 17 (1) ◽

pp. 13-25 ◽

Cited By ~ 18

Author(s):

Dino Celeda ◽

Klaus Aldinger ◽

Frank-Martin Haar ◽

Michael Hausmann ◽

Markus Durm ◽

...

Keyword(s):

Image Analysis ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Repetitive Dna ◽

Digital Image ◽

Digital Image Analysis ◽

Dna Probes

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Novel, small DNA probes that bind to tightly localized highly-reiterated sequences facilitate chromosome enumeration in interphase nuclei by in situ hybridization

Cancer Genetics and Cytogenetics ◽

10.1016/0165-4608(91)90370-a ◽

1991 ◽

Vol 56 (1) ◽

pp. 94

Author(s):

Heinz-Ulrich Weier ◽

Horst Zitzelsberger ◽

Mayumi Matsuta ◽

Morimasa Matsuta ◽

Joe W. Gray

Keyword(s):

In Situ Hybridization ◽

Dna Probes ◽

Interphase Nuclei

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Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing

Cytotechnology ◽

10.1007/bf00351116 ◽

1987 ◽

Vol 1 (1) ◽

pp. 13-17 ◽

Cited By ~ 1

Author(s):

E. Raimondi ◽

S. Scariolo ◽

P. Vagnarelli ◽

A. De Sario ◽

L. De Carli

Keyword(s):

In Situ Hybridization ◽

Repetitive Dna ◽

Cytogenetic Analysis ◽

Dna Probes ◽

Chromosome Variation ◽

Mutagenicity Testing ◽

Potential Applications

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A Simplified Combination of DNA Probe Preparation and Fluorescence in situ Hybridization

Zeitschrift für Naturforschung C ◽

10.1515/znc-1992-9-1017 ◽

1992 ◽

Vol 47 (9-10) ◽

pp. 739-747 ◽

Cited By ~ 9

Author(s):

Dino Celeda ◽

Ulrich Bettag ◽

Christoph Cremer

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Repetitive Dna ◽

Human Lymphocytes ◽

Dna Probes ◽

Dna Probe ◽

Nick Translation ◽

Metaphase Chromosomes ◽

Human Dna

Fluorescence in situ hybridization (FISH) has found widespread applications in cytogenetics. So far the standard protocols for probe amplification (and simultaneous labeling) by PCR, nick translation and in situ hybridization involve different buffer systems leading to a number of time consuming washing steps even before hybridization. In this manuscript we show a fast technique of a close combination of DNA probe preparation and in situ hybridization (ISH). This method was applied to metaphase chromosomes from human lymphocytes fixed on slides. Two specific repetitive DNA probes, the pUC 1.77 DNA probe and the DYZ 1 repetitive DNA fraction were used, amplified and labeled in different ways. Additional experiments with total genomic male human DNA as the DNA probe suggest that this method may be extended to a large variety of other probes. Moreover the ISH technique described does not require toxic denaturing agents, such as formamide.

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