Characterization of NADPH-cytochrome P-450 reductase in a mouse hepatoma cell line

1983 ◽  
Vol 18 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Gwen S. Duthu ◽  
Mark S. Nestor ◽  
Judith A. Berliner ◽  
Richard M. Philpot ◽  
Oliver Hankinson
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Nadph Cytochrome ◽  
Mouse Hepatoma ◽  
Cytochrome P 450

Structure of the rainbow trout metallothionein B gene and characterization of its metal-responsive region

1988 ◽  
Vol 8 (10) ◽  
pp. 4469-4476
Author(s):  
M Zafarullah ◽  
K Bonham ◽  
L Gedamu
Keyword(s):  
Rainbow Trout ◽  
Cell Line ◽  
Binding Sites ◽  
Transcription Initiation ◽  
Hepatoma Cell ◽  
Initiation Site ◽  
Hepatoma Cell Line ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene

The trout metallothionein (MT) genes consist of two members. We describe the structure of the first fish MT (tMT-B) gene which shows an overall resemblance but some remarkable differences with mammalian MT genes. The similarities included (i) tripartite structure of the gene, (ii) conservation of cysteine residues, and (iii) a TATAAA signal and two copies of metal-responsive elements (MREs). The differences consisted of (i) an AT-rich tMT-B promoter compared with highly GC-rich mammalian MT promoters and (ii) a lack of SP1-binding sites in the tMT-B promoter. Functional analysis of the tMT-B 5'-flanking region following fusion with the bacterial chloramphenicol acetyltransferase gene and its transfection into the rainbow trout hepatoma cell line revealed that sequences from positions -600 to +8 are sufficient for regulation by metals. Further deletion analyses of this fragment suggested that a minimum of 100 nucleotides upstream of the transcription initiation site are required for induction by cadmium and zinc. The tMT-B promoter was also functional in the human hepatoblastoma cell line, suggesting that an MT regulatory factor(s) is conserved in phylogenetically distant species like humans and fish.

Characterization of HCV-like particles produced in a human hepatoma cell line by a recombinant baculovirus

2006 ◽  
Vol 340 (1) ◽  
pp. 200-208 ◽  
Author(s):  
Eiko Matsuo ◽  
Hideki Tani ◽  
Chang kweng Lim ◽  
Yasumasa Komoda ◽  
Toru Okamoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Recombinant Baculovirus ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line

scholarly journals Structure of the rainbow trout metallothionein B gene and characterization of its metal-responsive region.

1988 ◽  
Vol 8 (10) ◽  
pp. 4469-4476 ◽  
Author(s):  
M Zafarullah ◽  
K Bonham ◽  
L Gedamu
Keyword(s):  
Rainbow Trout ◽  
Cell Line ◽  
Binding Sites ◽  
Transcription Initiation ◽  
Hepatoma Cell ◽  
Initiation Site ◽  
Hepatoma Cell Line ◽  
Flanking Region ◽  
Chloramphenicol Acetyltransferase Gene

The trout metallothionein (MT) genes consist of two members. We describe the structure of the first fish MT (tMT-B) gene which shows an overall resemblance but some remarkable differences with mammalian MT genes. The similarities included (i) tripartite structure of the gene, (ii) conservation of cysteine residues, and (iii) a TATAAA signal and two copies of metal-responsive elements (MREs). The differences consisted of (i) an AT-rich tMT-B promoter compared with highly GC-rich mammalian MT promoters and (ii) a lack of SP1-binding sites in the tMT-B promoter. Functional analysis of the tMT-B 5'-flanking region following fusion with the bacterial chloramphenicol acetyltransferase gene and its transfection into the rainbow trout hepatoma cell line revealed that sequences from positions -600 to +8 are sufficient for regulation by metals. Further deletion analyses of this fragment suggested that a minimum of 100 nucleotides upstream of the transcription initiation site are required for induction by cadmium and zinc. The tMT-B promoter was also functional in the human hepatoblastoma cell line, suggesting that an MT regulatory factor(s) is conserved in phylogenetically distant species like humans and fish.

scholarly journals Isolation and Characterization of Subpopulations of Rat Ascites Hepatoma Cell Line of AH109A with Different Metastatic Potentials

2003 ◽  
Vol 43 (1-3) ◽  
pp. 27-32
Author(s):  
Yutaka Miura ◽  
Miyako Ariga ◽  
Maiko Miyauchi ◽  
Katsuhiko Arai ◽  
Kazumi Yagasaki
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Ascites Hepatoma ◽  
Isolation And Characterization ◽  
Rat Ascites Hepatoma
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