Evidence against the involvement of adenosine-3′:5′ cyclic monophosphate in gibberellic acid-mediated growth as studied in Pharbitis nil

1975 ◽  
Vol 4 (4) ◽  
pp. 217-223 ◽  
Author(s):  
G.W.M. Barendse ◽  
G.T.M. Holtslag
Keyword(s):  
Gibberellic Acid ◽  
Pharbitis Nil ◽  
Cyclic Monophosphate

scholarly journals Regeneration of Pharbitis nil from immature embryos by somatic embryogenesis

2014 ◽  
Vol 66 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Alina Trejgell ◽  
Andrzej Tretyn
Keyword(s):  
Somatic Embryogenesis ◽  
Gibberellic Acid ◽  
Growth Medium ◽  
Somatic Embryos ◽  
Immature Embryos ◽  
Pharbitis Nil ◽  
Naphthaleneacetic Acid ◽  
Photoperiodic Induction ◽  
Root Region

Immature embryos of <em>Pharbitis nil</em> Chois. were used for the study. They were isolated from previously sterilized fruits and afterwards transferred to Murashige and Skoog (MS) growth medium with 0.8% agar. Immature embryos were cut across their axis. After 6-8 weeks of cultivation in the injury place (hypocotyl-root region) somatic embryos were formed. These embryos were isolated and each of them was transferred into a separate tube containing MS supplemented with naphthaleneacetic acid (NAA; in concentration 0.1 mg•dm<sup>-3</sup> ) and gibberellic acid (GA<sub>3</sub>; in concentration 0.5 mg•dm<sup>-3</sup>). Under these conditions about 26% of somatic embryos regenerated into complete plants. Two-three weeks after the photoperiodic induction flowers appeared on these plants. A few weeks after pollination normal seeds were developed from these flowers.

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