Purification and characterization of macrolide 2′-phosphotransferase type II from a strain of Escherichia coli highly resistant to macrolide antibiotics

1992 ◽  
Vol 97 (1-2) ◽  
pp. 89-94 ◽  
Author(s):  
M Kono
Keyword(s):  
Escherichia Coli ◽  
Macrolide Antibiotics ◽  
Type Ii ◽  
Purification And Characterization

scholarly journals Purification and Characterization of Recombinant Staphylococcus haemolyticus DNA Gyrase and Topoisomerase IV Expressed in Escherichia coli

2004 ◽  
Vol 48 (7) ◽  
pp. 2708-2711 ◽  
Author(s):  
Joel C. Bronstein ◽  
Stacey L. Olson ◽  
Kristin LeVier ◽  
Mark Tomilo ◽  
Peter C. Weber
Keyword(s):  
Escherichia Coli ◽  
Dna Gyrase ◽  
Type Ii ◽  
Topoisomerase Inhibitors ◽  
Purification And Characterization ◽  
Topoisomerase Iv ◽  
Staphylococcal Species ◽  
Staphylococcus Haemolyticus

ABSTRACT The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating type II topoisomerases of a coagulase-negative staphylococcal species.

Overproduction, Purification, and Characterization of β-1,3-Glucanase Type II in Escherichia coli

2001 ◽  
Vol 23 (2) ◽  
pp. 219-225 ◽  
Author(s):  
Oriana Salazar ◽  
Julia Molitor ◽  
M.Elena Lienqueo ◽  
Juan A. Asenjo
Keyword(s):  
Escherichia Coli ◽  
Type Ii ◽  
Purification And Characterization
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