Reversed-phase liquid chromatography of the opioid peptides. 3. Development of a microanalytical system for opioid peptides involving microbore liquid chromatography, post-column derivatization and laser-induced fluorescence detection

1992 ◽  
Vol 10 (10-12) ◽  
pp. 965-977 ◽  
Author(s):  
K.J. Dave ◽  
J.F. Stobaugh ◽  
T.M. Rossi ◽  
C.M. Riley
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Opioid Peptides ◽  
Laser Induced Fluorescence ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Laser Induced Fluorescence Detection ◽  
Phase Liquid ◽  
Microbore Liquid Chromatography

scholarly journals Multiresidue Determination of Fluoroquinolones in Eggs

2000 ◽  
Vol 83 (6) ◽  
pp. 1306-1312 ◽  
Author(s):  
Marilyn J Schneider ◽  
Dan J Donoghue
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Small Volume ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Trace Enrichment ◽  
On Line ◽  
Phase Liquid ◽  
Multiresidue Determination

Abstract A multiresidue method was developed for the determination of fluoroquinolones in eggs. Extraction of eggs with ammoniacal acetonitrile was followed by liquid–liquid defatting, solvent evaporation, and redissolution in a small volume of buffer. The fluoroquinolones were further purified by on-line microdialysis, concentrated on a trace enrichment column, and separated by reversed-phase liquid chromatography with fluorescence detection. Norfloxacin (NOR), ciprofloxacin (CIP), and sarafloxacin (SAR) were extracted from fortified eggs over a range of 2–200 μg/kg, with recoveries of 65.7–78.9%, 65.6–77.1%, and 67.6–110%, respectively. Enrofloxacin (ENRO) was extracted over a range of 1–100 μg/kg, with recoveries of 71.5–86.7%, whereas desethylene ciprofloxacin (DCIP) and danofloxacin (DANO) were extracted over a range of 0.2–20 μg/kg, with recoveries of 68.7–90.7% and 76.0–93.8%, respectively. The limits of quantitation for the 6 fluoroquinolones were as follows: DCIP and DANO, 0.3 μg/kg; ENRO, 1 μg/kg; NOR and CIP, 2 μg/kg; and SAR, 3 μg/kg. Both SAR and ENRO incurred eggs were also successfully analyzed using this method.

Liquid-chromatographic study of fluorescent compounds in hemodialysate solutions.

1985 ◽  
Vol 31 (1) ◽  
pp. 127-130 ◽  
Author(s):  
A L Barnett ◽  
H Veening
Keyword(s):  
Acetic Acid ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Reversed Phase ◽  
Chromatographic Study ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Liquid Chromatographic ◽  
Fluorescent Compounds ◽  
Indole 3 Acetic Acid

Abstract We have separated and identified three endogenous, naturally fluorescent substances in uremic hemodialysate by using reversed-phase liquid chromatography with fluorescence detection. Co-chromatography with authentic standards, monitoring peak shifts after enzymic treatment, and spectrofluorescence measurements were used to confirm the identity of indican, tryptophan, and indole-3-acetic acid. Concentrations of indican were about 1.5 those of tryptophan and considerably greater than those of indole-3-acetic acid in hemodialysate samples from 12 renal patients. These three compounds, as well as eight unidentified components, were consistently present in dialysate samples from each of the 12 patients.

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