Sympathetic neurons mediate developmental change in cardiac sodium channel gating through long-term neurotransmitter action

Neuron ◽  
1992 ◽  
Vol 9 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Ji-Fang Zhang ◽  
Richard B. Robinson ◽  
Steven A. Siegelbaum
Keyword(s):  
Sodium Channel ◽  
Developmental Change ◽  
Sympathetic Neurons ◽  
Channel Gating ◽  
Cardiac Sodium Channel

scholarly journals Modulation of Cardiac Sodium Channel Gating by Protein Kinase A Can Be Altered by Disease-linked Mutation

2003 ◽  
Vol 278 (47) ◽  
pp. 46718-46726 ◽  
Author(s):  
Michihiro Tateyama ◽  
Ilaria Rivolta ◽  
Colleen E. Clancy ◽  
Robert S. Kass
Keyword(s):  
Protein Kinase ◽  
Sodium Channel ◽  
Protein Kinase A ◽  
Channel Gating ◽  
Cardiac Sodium Channel ◽  
Kinase A

Two molecular transitions influence cardiac sodium channel gating

Science ◽  
1989 ◽  
Vol 244 (4902) ◽  
pp. 349-352 ◽  
Author(s):  
D. Yue ◽  
J. Lawrence ◽  
E Marban
Keyword(s):  
Sodium Channel ◽  
Channel Gating ◽  
Cardiac Sodium Channel

scholarly journals Calcium-Mediated Dual-Mode Regulation of Cardiac Sodium Channel Gating

2009 ◽  
Vol 104 (7) ◽  
pp. 870-878 ◽  
Author(s):  
Subrata Biswas ◽  
Deborah DiSilvestre ◽  
Yanli Tian ◽  
Victoria L. Halperin ◽  
Gordon F. Tomaselli
Keyword(s):  
Sodium Channel ◽  
Channel Gating ◽  
Dual Mode ◽  
Cardiac Sodium Channel

An analogue of cAMP mimics developmental change in neonatal rat ventricular myocyte sodium current kinetics

1996 ◽  
Vol 270 (1) ◽  
pp. H194-H199
Author(s):  
L. J. Lipka ◽  
S. A. Siegelbaum ◽  
R. B. Robinson ◽  
M. F. Berman
Keyword(s):  
Sodium Channel ◽  
Prolonged Exposure ◽  
Developmental Change ◽  
Sodium Current ◽  
Sympathetic Neurons ◽  
Neonatal Rat ◽  
Patch Clamp Technique ◽  
Inactivation Curve ◽  
Whole Cell ◽  
Cyclic Monophosphate

During development, the voltage dependence of single rat ventricular sodium channels shifts to more negative potentials. This shift is mimicked by coculture of neonatal myocytes with sympathetic neurons or by a 96-h exposure to 8-(4-chlorophenylthio) adenosine 3',5'-cyclic monophosphate (CPT-cAMP). The prolonged exposure to CPT-cAMP suggests that this is not a short-term modulatory effect on the sodium channel, but rather may reflect a trophic action. Here we examine the effect of CPT-cAMP using whole cell recording to investigate further the time period required for the effect. Sodium current was measured in a 50 mM NaCl bath solution at 20 +/- 1 degree C using the whole cell patch-clamp technique after exposure of myocytes to CPT-cAMP (0.25 mM) for 0,0.5,20, or 24 h. The relationship between the time constant of decay (tauh) of the sodium current and test voltage (V1) showed a shift to more hyperpolarizing voltages after exposure to CPT-cAMP for 24 h. In addition, the midpoint of the steady-state inactivation curve (V 1/2) was shifted from -75.8 +/- 1.1 mV (0-h exposure) to -83.3 +/- 1.6 mV (24-h exposure) (P < 0.05). Exposure for 0.5 h to CPT-cAMP did not alter the tauh or V 1/2 of the sodium current. However, exposure to CPT-cAMP for 20 h, followed by a 4-h washout period, produced an effect similar to that of the 24-h exposure. Thus the lack of effect of acute (0.5 h) exposure to CPT-cAMP and the persistence of the effect after washout of CPT-cAMP for 4 h suggest that adenosine 3',5'-cyclic monophosphate may play a trophic role in sodium channel development.

Unusual Intranuclear Structures in Chick Sympathetic Neurons

1967 ◽  
Vol 25 ◽  
pp. 188-189
Author(s):  
E. B. Masurovsky ◽  
H. H. Benitez ◽  
M. R. Murray
Keyword(s):  
Electron Microscope ◽  
Sympathetic Neurons ◽  
Uranyl Acetate ◽  
Sympathetic Ganglia ◽  
Lead Citrate ◽  
Thin Sections ◽  
Cns Neurons ◽  
Mammalian Cns

Recent light- and electron microscope studies concerned with the effects of D2O on the development of chick sympathetic ganglia in long-term, organized culture revealed the presence of rod-like fibrillar formations, and associated granulofibrillar bodies, in the nuclei of control and deuterated neurons. Similar fibrillar formations have been reported in the nuclei of certain mammalian CNS neurons; however, related granulofibrillar bodies have not been previously described. Both kinds of intranuclear structures are observed in cultures fixed either in veronal acetate-buffered 2%OsO4 (pH 7. 4), or in 3.5% glutaraldehyde followed by post-osmication. Thin sections from such Epon-embedded cultures were stained with ethanolic uranyl acetate and basic lead citrate for viewing in the electron microscope.

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