New results on spin determination of nanosatellite BLITS from High Repetition Rate SLR data

2013 ◽  
Vol 51 (5) ◽  
pp. 912-916 ◽  
Author(s):  
D. Kucharski ◽  
G. Kirchner ◽  
H.-C. Lim ◽  
F. Koidl
IUCrJ ◽  
2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Suraj Pandey ◽  
George Calvey ◽  
Andrea M. Katz ◽  
Tek Narsingh Malla ◽  
Faisal H. M. Koua ◽  
...  

Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution using the most advanced X-ray source currently available: the European XFEL (EuXFEL). The high repetition rate of the EuXFEL combined with our mix-and-inject technology enables the initial phase of ceftriaxone binding to the Mycobacterium tuberculosis β-lactamase to be followed using time-resolved crystallography in real time. It is shown how a diffusion coefficient in enzyme crystals can be derived directly from the X-ray data, enabling the determination of ligand and enzyme–ligand concentrations at any position in the crystal volume as a function of time. In addition, the structure of the irreversible inhibitor sulbactam bound to the enzyme at a 66 ms time delay after mixing is described. This demonstrates that the EuXFEL can be used as an important tool for biomedically relevant research.


1999 ◽  
Vol 5 (6) ◽  
pp. 1495-1501 ◽  
Author(s):  
I.A. Artioukov ◽  
B.R. Benware ◽  
J.J. Rocca ◽  
M. Forsythe ◽  
Yu.A. Uspenskii ◽  
...  

Author(s):  
Liqiang Zhou ◽  
Chen Wei ◽  
Dongsheng Wang ◽  
Hao Chi ◽  
Le Zheng ◽  
...  

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