Limitations of the 15N natural abundance method for estimating biological nitrogen fixation in Amazonian forest legumes

2004 ◽  
Vol 5 (6) ◽  
pp. 567-580 ◽  
Author(s):  
Christoph Gehring ◽  
Paul L.G. Vlek
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
Amazonian Forest ◽  
15N Natural Abundance Method ◽  
Biological Nitrogen

Differences in contribution of biological nitrogen fixation to yield performance of common bean cultivars as assessed by the 15N natural abundance technique

2020 ◽  
Vol 454 (1-2) ◽  
pp. 327-341
Author(s):  
Rafael Sanches Pacheco ◽  
Robert Michael Boddey ◽  
Bruno José Rodrigues Alves ◽  
Enderson Petrônio de Brito Ferreira ◽  
Rosângela Straliotto ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Common Bean ◽  
Biological Nitrogen Fixation ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
Yield Performance ◽  
Biological Nitrogen

scholarly journals The 15N natural abundance technique to assess the potential of biological nitrogen fixation (BNF) in some important C4 grasses

2017 ◽  
Vol 11 (12) ◽  
pp. 1559-1564 ◽  
Author(s):  
Eric Xavier de Carvalho ◽  
◽  
Rômulo Simões Cezar Menezes ◽  
Ana Dolores Santiago de Freitas ◽  
Everardo Valadares de Sá Barretto Sampaio ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
C4 Grasses ◽  
Biological Nitrogen

Symbiotic nitrogen fixation in eight Acacia senegal provenances in dryland clays of the Blue Nile Sudan estimated by the 15N natural abundance method

2005 ◽  
Vol 275 (1-2) ◽  
pp. 261-269 ◽  
Author(s):  
El Amin Yousif Raddad ◽  
Ahmed Ali Salih ◽  
Mohamed Ahmed El Fadl ◽  
Vesa Kaarakka ◽  
Olavi Luukkanen
Keyword(s):  
Nitrogen Fixation ◽  
Symbiotic Nitrogen Fixation ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
Acacia Senegal ◽  
Blue Nile ◽  
15N Natural Abundance Method

Nitrogen fixation in chickpea. II. Comparison of 15N enrichment and 15N natural abundance methods for estimating nitrogen fixation

1995 ◽  
Vol 46 (1) ◽  
pp. 225 ◽  
Author(s):  
JA Doughton ◽  
PG Saffigna ◽  
I Vallis ◽  
RJ Mayer
Keyword(s):  
Nitrogen Fixation ◽  
N2 Fixation ◽  
Full Range ◽  
15N Natural Abundance ◽  
Natural Abundance ◽  
Enrichment Method ◽  
Mineral N ◽  
Crop Establishment ◽  
15N Enrichment ◽  
15N Natural Abundance Method

The 15N enrichment and 15N natural abundance methods for estimating N2 fixation in chickpea were compared over a range of soil NO3-N levels at crop establishment varying from 10 to 326 kg N/ha (0-120 cm depth). Barley was used as a non-N2 fixing control crop. Both methods estimated reduced N2 fixation as soil NO3-N levels at crop establishment increased. Similar estimates of % N2 fixation were obtained at high values, but at low values the enrichment method gave lower estimates, some of which were negative. The 15N natural abundance method provided realistic estimates of % N2 fixation across all soil N03-N levels at crop establishment. An asymptotic curve described a close ( R2 = 0.95) relationship between these factors. Standard errors of estimates of means for the 15N natural abundance method remained acceptable and relatively stable over the full range of measurements; however, with the 15N enrichment method they became unacceptably large at low values of % N2 fixation. These large errors may have been partly due to legume and control plants assimilating mineral N of differing 15N enrichment. High mineral N levels associated with low values of % N2 fixation were also shown to reduce reliability of N2 fixation values estimated by the 15N enrichment method. These errors caused potentially greater inaccuracy at low values of % N2 fixation than at high values. To compare N2 fixation means statistically, transformations were necessary to stabilize variance and to impart lower weightings to plots with low values of % N2 fixation.

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