scholarly journals Ca2+-Sensitivity and Elementary Steps of the Cross-Bridge Cycle in Papillary Muscle Fibers from the Troponin C (TnC)-A8V Knock-In Mouse, which Exhibits Hypertrophic Cardiomyopathy (HCM)

2016 ◽  
Vol 110 (3) ◽  
pp. 464a-465a
Author(s):  
Masataka Kawai ◽  
Tarek Karam ◽  
Jose R. Pinto
Keyword(s):  
Hypertrophic Cardiomyopathy ◽  
Papillary Muscle ◽  
Muscle Fibers ◽  
Troponin C ◽  
Elementary Steps ◽  
Cross Bridge ◽  
The Cross

Evaluation of the cross-bridge-dependent change in the Ca2+ affinity of troponin C in aequorin-injected ferret ventricular muscles

Cell Calcium ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 153-162 ◽  
Author(s):  
Tetsuya Ishikawa ◽  
Jin O-Uchi ◽  
Seibu Mochizuki ◽  
Satoshi Kurihara
Keyword(s):  
Troponin C ◽  
Cross Bridge ◽  
Dependent Change ◽  
The Cross

scholarly journals Cross-Bridge Kinetics in Rat Papillary Muscle Fibers that Carry α-MHC and β-MHC by Sinusoidal Analysis

2016 ◽  
Vol 110 (3) ◽  
pp. 294a-295a
Author(s):  
Tarek S. Karam ◽  
John J. Michael ◽  
Chandra Murali ◽  
Masataka Kawai
Keyword(s):  
Papillary Muscle ◽  
Muscle Fibers ◽  
Sinusoidal Analysis ◽  
Cross Bridge ◽  
Rat Papillary Muscle

BDM affects nucleotide binding and force generation steps of the cross-bridge cycle in rabbit psoas muscle fibers

1994 ◽  
Vol 266 (2) ◽  
pp. C437-C447 ◽  
Author(s):  
Y. Zhao ◽  
M. Kawai
Keyword(s):  
Equilibrium Constants ◽  
Muscle Fibers ◽  
Psoas Muscle ◽  
Isometric Tension ◽  
Activation Mechanism ◽  
Phosphate Binding ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
The Cross ◽  
Cross Bridges

The effect of 2,3-butanedione monoxime (BDM) on elementary steps of the cross-bridge cycle was studied with the sinusoidal analysis technique in skinned rabbit psoas muscle fibers. Our results showed that isometric tension and stiffness decreased progressively with an increase in the BDM concentration. The MgATP and MgADP binding constants increased 27 and 6 times, respectively, when BDM was increased from 0 to 18 mM, whereas the phosphate binding constant did not change significantly. The equilibrium constants of the ATP isomerization and detachment step were not sensitive to BDM, whereas the equilibrium constant of the attachment (power stroke) step decreased with BDM. Thus, in the presence of BDM, the number of attached cross bridges decreases; more cross bridges accumulate in the detached state, causing isometric tension and stiffness to decline. However, our detailed analysis shows that the decrease in the number of attached cross bridges is approximately 40%, which is not adequate to account for the 84% decrease in the isometric tension when 18 mM BDM was present. Therefore we suggest that a thin-filament activation mechanism is also affected by BDM.

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