Isolation and characterization of Heat Shock Protein 100-Batu1 from Toxoplasma gondii RH strain

2015 ◽  
Vol 153 ◽  
pp. 91-97
Author(s):  
Kübra Açıkalın Coşkun ◽  
Yusuf Tutar
2005 ◽  
Vol 4 (12) ◽  
pp. 1990-1997 ◽  
Author(s):  
N. de Miguel ◽  
P. C. Echeverria ◽  
S. O. Angel

ABSTRACT The results of this study describe the identification and characterization of the Toxoplasma gondii α-crystallin/small heat shock protein (sHsp) family. By database (www.toxodb.org ) search, five parasite sHsps (Hsp20, Hsp21, Hsp28, Hsp29, and the previously characterized Hsp30/Bag1) were identified. As expected, they share the homologous α-crystallin domain, which is the key characteristic of sHsps. However, the N-terminal segment of each protein contains unique characteristics in size and sequence. Most T. gondii sHsps are constitutively expressed in tachyzoites and fully differentiated bradyzoites, with the exception of Hsp30/Bag1. Interestingly, by subcellular localization we observed that T. gondii sHsps are located in different compartments. Hsp20 is located at the apical end of the cell, Hsp28 is located inside the mitochondrion, Hsp29 showed a membrane-associated labeling, and Hsp21 appeared throughout the cytosol of the parasites. These particular differences in the immunostaining patterns suggest that their targets and functions might be different.


Plant Science ◽  
2006 ◽  
Vol 170 (5) ◽  
pp. 976-985 ◽  
Author(s):  
Nai-yu Liu ◽  
Swee-suak Ko ◽  
Kuo-Chen Yeh ◽  
Yee-yung Charng

1991 ◽  
Vol 96 (4) ◽  
pp. 1268-1276 ◽  
Author(s):  
Paul S. Dietrich ◽  
Robert A. Bouchard ◽  
Elena Silva Casey ◽  
Ralph M. Sinibaldi

Euphytica ◽  
2006 ◽  
Vol 147 (3) ◽  
pp. 343-351 ◽  
Author(s):  
Kosmas A. Sotirios ◽  
Alexandros Argyrokastritis ◽  
Michael Loukas ◽  
Elias Eliopoulos ◽  
Spyros Tsakas ◽  
...  

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