Agrobacterium tumefaciens -mediated transformation of the entomopathogenic fungus Nomuraea rileyi

2015 ◽  
Vol 83 ◽  
pp. 19-25 ◽  
Author(s):  
Changwen Shao ◽  
Youping Yin ◽  
Zhaoran Qi ◽  
Ren Li ◽  
Zhangyong Song ◽  
...  
1993 ◽  
Vol 122 (3) ◽  
pp. 149-152 ◽  
Author(s):  
G. N. El-Sayed ◽  
C. M. Ignoffo ◽  
T. D. Leathers ◽  
S. C. Gupta

2007 ◽  
Vol 49 ◽  
pp. 67-68
Author(s):  
Naofumi Hozumi ◽  
Yoko Kawamura ◽  
Yoshinari Enami

2006 ◽  
Vol 0 (0) ◽  
pp. 060329075718009 ◽  
Author(s):  
I.G.P. Lima ◽  
R.T.D. Duarte ◽  
L. Furlaneto ◽  
C.H. Baroni ◽  
M.H.P. Fungaro ◽  
...  

1978 ◽  
Vol 7 (5) ◽  
pp. 724-727 ◽  
Author(s):  
C. M. Ignoffo ◽  
Clemente Garcia ◽  
D. L. Hostetter ◽  
R. E. Pinnell

1981 ◽  
Vol 27 (3) ◽  
pp. 364-366 ◽  
Author(s):  
D. J. Joslyn ◽  
D. G. Boucias

The zymogram technique has been applied to three pathotypes of the entomopathogenic fungus Nomuraea rileyi. Isozyme profiles of isolates from Heliothis zea, Pseudoplusia includens, and Anticarsia gemmatalis were compared for 17 enzymes of known metabolic function. Electrophoretic data supported the taxonomic differences inferred for the three pathotypes based on host specificity. The isolate from A. gemmatalis was found consistently to be the most distinct.This study demonstrates that isozyme analysis may be used to distinguish closely related fungal isolates of N. rileyi.


2002 ◽  
Vol 45 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Raul Riveros Gonzalez ◽  
Cláudio Luiz Messias ◽  
João Lúcio Azevedo ◽  
Neiva Monteiro de Barros

The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.


1984 ◽  
Vol 43 (2) ◽  
pp. 254-258 ◽  
Author(s):  
Drion G. Boucias ◽  
Dawn L. Brasaemle ◽  
James L. Nation

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