Immobilizing Topoisomerase I on a Surface Plasmon Resonance Biosensor Chip to Screen for Top1-DNA Cleavable Complex Inhibitors

2010 ◽  
Vol 49 ◽  
pp. S201-S202
Author(s):  
Hsiang Ping Tsai ◽  
Jaulang Hwang ◽  
Chun Mao Lin
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Topoisomerase I ◽  
Surface Plasmon Resonance Biosensor ◽  
Biosensor Chip ◽  
Cleavable Complex

scholarly journals Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors

2010 ◽  
Vol 17 (1) ◽  
pp. 49 ◽  
Author(s):  
Hsiang-Ping Tsai ◽  
Li-Wei Lin ◽  
Zhi-Yang Lai ◽  
Jui-Yu Wu ◽  
Chiao-En Chen ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Topoisomerase I ◽  
Surface Plasmon Resonance Biosensor ◽  
Biosensor Chip

scholarly journals A surface plasmon resonance biosensor for direct detection of the rabies virus

2012 ◽  
Vol 81 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Jing Xu ◽  
Jia-yu Wan ◽  
Song-tao Yang ◽  
Shou-feng Zhang ◽  
Na Xu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Rabies Virus ◽  
Surface Plasmon ◽  
Specific Antibody ◽  
Plasmon Resonance ◽  
Fluorescent Antibody ◽  
Test Surface ◽  
N Protein ◽  
Surface Plasmon Resonance Biosensor ◽  
Biosensor Chip

A surface plasmon resonance biosensor chip was constructed for detection of rabies virus. For the construction of the biosensor chip, N protein specific antibody and N protein specific antibody combined with G protein specific antibody of rabies virus were linked on two different flow cells on one CM5 chip, respectively. The chip was tested for the detection of rabies virus antigens using the crude extract of rabies virus from infected BHK cell strain culture. Tenfold serial dilutions of SRV9 strain virus-infected cell cultures were tested by the biosensor chip to establish the detection limit. The limit detection was approximately 70 pg/ml of nucleoprotein and glycoprotein. The biosensor chip developed in this study was employed for the detection of rabies virus in five suspect infectious specimens of brain tissue from guinea pigs; the results were compared by fluorescent antibody test. Surface plasmon resonance biosensor chip could be a useful automatic tool for prompt detection of rabies virus infection.

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