Eriocheir sinensis microRNA-7 targets crab Myd88 to enhance white spot syndrome virus replication

2018 ◽  
Vol 79 ◽  
pp. 274-283 ◽  
Author(s):  
Ying Huang ◽  
Wen Wang ◽  
Zhiqiang Xu ◽  
Jianlin Pan ◽  
Zhe Zhao ◽  
...  
2015 ◽  
Vol 220 ◽  
pp. 49-54 ◽  
Author(s):  
Zhengfeng Ding ◽  
Yufeng Yao ◽  
Fengxiang Zhang ◽  
Jinjuan Wan ◽  
Mengling Sun ◽  
...  

2019 ◽  
Vol 93 ◽  
pp. 313-321 ◽  
Author(s):  
Menghao Wei ◽  
Yueling Zhang ◽  
Jude Juventus Aweya ◽  
Fan Wang ◽  
Shengkang Li ◽  
...  

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91930 ◽  
Author(s):  
Tipachai Vatanavicharn ◽  
Adisak Prapavorarat ◽  
Phattarunda Jaree ◽  
Kunlaya Somboonwiwat ◽  
Anchalee Tassanakajon

2016 ◽  
Vol 48 (5) ◽  
pp. 2528-2537 ◽  
Author(s):  
Zhengfeng Ding ◽  
Shaoming Wang ◽  
Xueshen Zhu ◽  
Jianlin Pan ◽  
Hui Xue

2009 ◽  
Vol 90 (6) ◽  
pp. 1483-1490 ◽  
Author(s):  
Fang He ◽  
Syed Musthaq Syed ◽  
A. S. Sahul Hameed ◽  
Jimmy Kwang

The E3 ligase WSSV222 of white spot syndrome virus (WSSV) is involved in anti-apoptosis regulation by ubiquitin-mediated degradation of tumour suppressor-like protein (TSL), a shrimp tumour suppressor. In the present study, WSSV222 gene expression was silenced by using specific small interfering RNA (siRNA) in Sf9 and BHK cells. Based on the results of the in vitro silencing, WSSV-challenged shrimp were treated with anti-WSSV222 siRNA to knock down WSSV222 protein expression. The survival rate of shrimp and the efficiency of WSSV replication were assessed to evaluate the efficacy of anti-WSSV222 siRNA in regulating WSSV infection in shrimp. The anti-WSSV222 siRNA reduced the cumulative mortality in shrimp challenged with 103 copies of WSSV and delayed the mean time to death in shrimp challenged with the higher dose of 106 copies. The results of real-time quantitative PCR showed that virus replication was delayed and reduced in WSSV-challenged shrimp treated with anti-WSSV222 siRNA in comparison with challenged shrimp treated with random-control siRNA. Co-immunoprecipitation assays revealed that WSSV222 silencing inhibited the degradation of TSL in WSSV-challenged shrimp, indicating the requirement for WSSV222 for efficient replication of WSSV in shrimp.


2009 ◽  
Vol 145 (1) ◽  
pp. 127-133 ◽  
Author(s):  
Pongsopee Attasart ◽  
Rossukon Kaewkhaw ◽  
Chaweewan Chimwai ◽  
Ukrit Kongphom ◽  
Orathai Namramoon ◽  
...  

2011 ◽  
Vol 85 (7) ◽  
pp. 3517-3525 ◽  
Author(s):  
Y.-R. Lin ◽  
H.-C. Hung ◽  
J.-H. Leu ◽  
H.-C. Wang ◽  
G.-H. Kou ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document