Normothermic Donor Lung Preservation Using the Organ Care System Significantly Reduces Ischemia/Reperfusion Injury by Promoting Cytokine Antagonists

2017 ◽  
Vol 36 (4) ◽  
pp. S69
Author(s):  
B. Wiegmann ◽  
C. Falk ◽  
M. Seyda ◽  
C. Neudoerfl ◽  
C. Kühn ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Lung Preservation ◽  
Cytokine Antagonists ◽  
Donor Lung ◽  
Care System

Normothermic donor lung preservation with portable evlp significantly reduces ischemia/reperfusion injury in lung recipients by promoting cytokine antagonists

Cryobiology ◽  
2018 ◽  
Vol 81 ◽  
pp. 227-228
Author(s):  
Christine S. Falk ◽  
Bettina Wiegmann ◽  
Ramon Bellmas Sanz ◽  
Christine Neudörfl ◽  
Christian Kühn ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Lung Preservation ◽  
Cytokine Antagonists ◽  
Donor Lung

Pilot Trial of Extended Hypothermic Lung Preservation to Analyze Ischemia-reperfusion Injury in Pigs

2021 ◽  
Vol 57 (7) ◽  
pp. 479-489
Author(s):  
Amaia Ojanguren ◽  
Maite Santamaría ◽  
Lucía Milla-Collado ◽  
Carlos Fraile ◽  
Sonia Gatius-Calderó ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Pilot Trial ◽  
Lung Preservation

5-HT Receptor Antagonism Attenuates the Ischemia-Reperfusion Injury After Rabbit Lung Preservation

2015 ◽  
Vol 47 (6) ◽  
pp. 1653-1656 ◽  
Author(s):  
J.L. Arreola-Ramírez ◽  
J. Alquicira-Mireles ◽  
P.E. Morales-Hernández ◽  
M.H. Vargas ◽  
J. Villalba-Caloca ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Rabbit Lung ◽  
Receptor Antagonism ◽  
Lung Preservation

Rapid postmortem ventilation improves donor lung viability by extending the tolerable warm ischemic time after cardiac death in mice

2021 ◽  
Author(s):  
Junyi Yu ◽  
Che Xu ◽  
Janet S. Lee ◽  
Jonathan K. Alder ◽  
Zongmei Wen ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Cardiac Death ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Lung Injury Score ◽  
Ischemic Time ◽  
Ischemia Time ◽  
Donor Lung

Uncontrolled donation after cardiac death (uDCD) contributes little to ameliorating donor lung shortage due to rapidly progressive warm ischemia after circulatory arrest. Here, we demonstrated non-hypoxia improves donor lung viability in a novel uDCD lung transplant model undergoing rapid ventilation after cardiac death and compared the evolution of ischemia-reperfusion injury in mice that underwent pulmonary artery ligation (PAL). The tolerable warm ischemia time at 37ºC was initially determined in mice using a modified PAL model. The donor lung following PAL was also transplanted into syngeneic mice and compared to those that underwent rapid ventilation or no ventilation at 37ºC prior to transplantation. Twenty-four hours following reperfusion, lung histology, PaO2/FIO2 ratio, and inflammatory mediators were measured. Four hours of PAL had little impact on PaO2/FIO2 ratio and acute lung injury score in contrast to significant injury induced by 5 hours of PAL. Four-hour PAL lungs showed an early myeloid-dominant inflammatory signature when compared to naïve lungs and substantially injured five-hour PAL lungs. In the context of transplantation, unventilated donor lungs showed severe injury after reperfusion, whereas ventilated donor lungs showed minimal changes in PaO2/FIO2 ratio, histologic score, and expression of inflammatory markers. Taken together, the tolerable warm ischemia time of murine lungs at 37oC can be extended by maintaining alveolar ventilation for up to 4 hours. Non-hypoxic lung warm ischemia-reperfusion injury shows an early transcriptional signature of myeloid cell recruitment and extracellular matrix proteolysis prior to blood-gas barrier dysfunction and significant tissue damage.

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