Structure and gene cluster of the K125 capsular polysaccharide from Acinetobacter baumannii MAR13-1452

2018 ◽  
Vol 117 ◽  
pp. 1195-1199 ◽  
Author(s):  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Andrei S. Dmitrenok ◽  
Anastasia V. Popova ◽  
Dmitry A. Shagin ◽  
...  
2020 ◽  
Vol 85 (2) ◽  
pp. 241-247 ◽  
Author(s):  
S. M. Cahill ◽  
N. P. Arbatsky ◽  
A. S. Shashkov ◽  
M. M. Shneider ◽  
A. V. Popova ◽  
...  

2017 ◽  
Vol 82 (4) ◽  
pp. 483-489 ◽  
Author(s):  
A. A. Kasimova ◽  
M. M. Shneider ◽  
N. P. Arbatsky ◽  
A. V. Popova ◽  
A. S. Shashkov ◽  
...  

2015 ◽  
Vol 413 ◽  
pp. 12-15 ◽  
Author(s):  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Johanna J. Kenyon ◽  
Alexander S. Shashkov ◽  
Anastasiya V. Popova ◽  
...  

Microbiology ◽  
2016 ◽  
Vol 162 (8) ◽  
pp. 1479-1489 ◽  
Author(s):  
Johanna J. Kenyon ◽  
Mikhail M. Shneider ◽  
Sofya N. Senchenkova ◽  
Alexander S. Shashkov ◽  
Maria N. Siniagina ◽  
...  

2021 ◽  
pp. 108439
Author(s):  
Nikolay P. Arbatsky ◽  
Anastasiya V. Popova ◽  
Mikhail M. Shneider ◽  
Alexander S. Shashkov ◽  
Ruth M. Hall ◽  
...  

2016 ◽  
Vol 434 ◽  
pp. 12-17 ◽  
Author(s):  
Johanna J. Kenyon ◽  
Immacolata Speciale ◽  
Ruth M. Hall ◽  
Cristina De Castro

2019 ◽  
Vol 128 ◽  
pp. 101-106 ◽  
Author(s):  
Johanna J. Kenyon ◽  
Nikolay P. Arbatsky ◽  
Emma L. Sweeney ◽  
Alexander S. Shashkov ◽  
Mikhail M. Shneider ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Woo Cheol Lee ◽  
Sungjae Choi ◽  
Ahjin Jang ◽  
Kkabi Son ◽  
Yangmee Kim

AbstractSome Gram-negative bacteria harbor lipids with aryl polyene (APE) moieties. Biosynthesis gene clusters (BGCs) for APE biosynthesis exhibit striking similarities with fatty acid synthase (FAS) genes. Despite their broad distribution among pathogenic and symbiotic bacteria, the detailed roles of the metabolic products of APE gene clusters are unclear. Here, we determined the crystal structures of the β-ketoacyl-acyl carrier protein (ACP) reductase ApeQ produced by an APE gene cluster from clinically isolated virulent Acinetobacter baumannii in two states (bound and unbound to NADPH). An in vitro visible absorption spectrum assay of the APE polyene moiety revealed that the β-ketoacyl-ACP reductase FabG from the A. baumannii FAS gene cluster cannot be substituted for ApeQ in APE biosynthesis. Comparison with the FabG structure exhibited distinct surface electrostatic potential profiles for ApeQ, suggesting a positively charged arginine patch as the cognate ACP-binding site. Binding modeling for the aryl group predicted that Leu185 (Phe183 in FabG) in ApeQ is responsible for 4-benzoyl moiety recognition. Isothermal titration and arginine patch mutagenesis experiments corroborated these results. These structure–function insights of a unique reductase in the APE BGC in comparison with FAS provide new directions for elucidating host–pathogen interaction mechanisms and novel antibiotics discovery.


2014 ◽  
Vol 391 ◽  
pp. 89-92 ◽  
Author(s):  
Sof’ya N. Senchenkova ◽  
Alexander S. Shashkov ◽  
Mikhail M. Shneider ◽  
Nikolay P. Arbatsky ◽  
Anastasiya V. Popova ◽  
...  

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