Predominance of oleanane-type ginsenoside R0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus C.A. Meyer

2022 ◽  
Vol 177 ◽  
pp. 114417
Author(s):  
Elena S. Glagoleva ◽  
Svetlana V. Konstantinova ◽  
Dmitry V. Kochkin ◽  
Vladimir Ossipov ◽  
Maria V. Titova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Panax Japonicus

The occurrence of gypenoside XVII in suspension cell culture of ginseng Panax japonicus var. repens

2012 ◽  
Vol 442 (1) ◽  
pp. 42-45
Author(s):  
D. V. Kochkin ◽  
G. P. Zaitsev ◽  
V. V. Kachala ◽  
A. O. Chizhov ◽  
E. V. Demidova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Panax Japonicus

Respiration activity of suspension cell culture of Polyscias filicifolia bailey, Stephania glabra (Roxb.) miers, and Dioscorea deltoidea wall

2011 ◽  
Vol 47 (1) ◽  
pp. 87-92 ◽  
Author(s):  
M. V. Titova ◽  
E. A. Berkovich ◽  
O. V. Reshetnyak ◽  
I. E. Kulichenko ◽  
A. V. Oreshnikov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Respiration Activity ◽  
Dioscorea Deltoidea

scholarly journals Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 671 ◽  
Author(s):  
Pucker ◽  
Rückert ◽  
Stracke ◽  
Viehöver ◽  
Kalinowski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Arabidopsis Thaliana ◽  
Large Scale ◽  
Suspension Cell ◽  
Reference Sequence ◽  
Model Organisms ◽  
Suspension Cell Culture ◽  
Single Nucleotide Variants ◽  
Single Nucleotide ◽  
Haploid Genome Size

Arabidopsis thaliana is one of the best studied plant model organisms. Besides cultivation in greenhouses, cells of this plant can also be propagated in suspension cell culture. At7 is one such cell line that was established about 25 years ago. Here, we report the sequencing and the analysis of the At7 genome. Large scale duplications and deletions compared to the Columbia-0 (Col-0) reference sequence were detected. The number of deletions exceeds the number of insertions, thus indicating that a haploid genome size reduction is ongoing. Patterns of small sequence variants differ from the ones observed between A. thaliana accessions, e.g., the number of single nucleotide variants matches the number of insertions/deletions. RNA-Seq analysis reveals that disrupted alleles are less frequent in the transcriptome than the native ones.

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