Vaccination of Porcine Circovirus type 2 (PCV2)-infected Sows against Porcine Parvovirus (PPV) and Erysipelas: Effect on Post-weaning Multisystemic Wasting Syndrome (PMWS) and on PCV2 Genome Load in the Offspring

2007 ◽  
Vol 136 (2-3) ◽  
pp. 133-144 ◽  
Author(s):  
N. Rose ◽  
P. Blanchard ◽  
R. Cariolet ◽  
B. Grasland ◽  
N. Amenna ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Porcine Parvovirus ◽  
Wasting Syndrome ◽  
Pcv2 Genome

scholarly journals Diagnosis of post-weaning multisystemic wasting syndrome in pigs in Brazil caused by porcine circovirus type 2

2003 ◽  
Vol 55 (5) ◽  
pp. 522-527 ◽  
Author(s):  
J.R. Ciacci-Zanella ◽  
N. Morés
Keyword(s):  
Clinical Signs ◽  
Rflp Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Nested Polymerase Chain Reaction ◽  
Wasting Syndrome ◽  
Thymus Atrophy ◽  
Granulomatous Lymphadenitis

This report describes the first preliminary characterization of porcine circovirus type 2 (PCV2) isolates from pigs affected with post-weaning multisystemic wasting syndrome (PMWS) in Brazil. Diseased pigs were examined at necropsy and by histopathology. Macroscopic and microscopic analyses revealed lesions reported to be typical of PMWS, which included, respectively, emaciation, enlargement of lymph nodes, thymus atrophy and interstitial pneumonia, and granulomatous lymphadenitis with syncytial cells, among others. Using nested polymerase chain reaction (PCR) or imunoperoxidase it was possible to detected DNA or antigen of PCV2, respectively. The PCR' s amplified fragment could be differentiated from PCV1 and PCV2 from one another by restriction fragment length polymorphism (RFLP) analysis. PCV2 DNA was detected in 70% (14/20) of samples of pigs with clinical signs and lesions associated with PMWS. This study shows that PCV2 is associated with lesions and symptoms indicative of PMWS in pigs. It is also shown that the Brazilian PCV2 isolates may have variation in their genome.

Infection, excretion and seroconversion dynamics of porcine circovirus type 2 (PCV2) in pigs from post-weaning multisystemic wasting syndrome (PMWS) affected farms in Spain and Denmark

2009 ◽  
Vol 135 (3-4) ◽  
pp. 272-282 ◽  
Author(s):  
Llorenç Grau-Roma ◽  
Charlotte K. Hjulsager ◽  
Marina Sibila ◽  
Charlotte S. Kristensen ◽  
Sergio López-Soria ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Wasting Syndrome

scholarly journals Conformational Changes and Nuclear Entry of Porcine Circovirus without Disassembly

2019 ◽  
Vol 93 (20) ◽  
Author(s):  
Huijuan Wang ◽  
Kailun Zhang ◽  
Cui Lin ◽  
Jianwei Zhou ◽  
Yulan Jin ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Viral Genome ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Nuclear Targeting ◽  
Early Endosomes ◽  
Pcv2 Genome ◽  
Infectious Cycle

ABSTRACT A relatively stable and flexible capsid is critical to the viral life cycle. However, the capsid dynamics and cytosol trafficking of porcine circovirus type 2 (PCV2) during its infectious cycle are poorly understood. Here, we report the structural stability and conformation flexibility of PCV2 virions by genome labeling and the use of three monoclonal antibodies (MAbs) against the native capsid of PCV2. Genome labeling showed that the infectivity of the PCV2 virion was not affected by conjugation with deoxy-5-ethynylcytidine (EdC). Heat stability experiments indicated that PCV2 capsids started to disassemble at 65°C, causing binding incompetence for all antibodies, and the viral genome was released without capsid disassembly upon heating at 60°C. Antibody binding experiments with PCV2 showed that residues 186 to 192 were concealed in the early endosomes of epithelial PK-15 and monocytic 3D4/31 cells with or without chloroquine treatment and then exposed in PK-15 cytosol and the 3D4/31 nucleus. Viral propagation and localization experiments showed that PCV2 replication and cytosol trafficking were not significantly affected by microtubule depolymerization in monocytic 3D4/31 cells treated with nocodazole. These findings demonstrated that nuclear targeting of viral capsids involved conformational changes, the PCV2 genome was released from the assembled capsid, and the transit of PCV2 particles was independent of microtubules in 3D4/31 cells. IMPORTANCE Circovirus is the smallest virus known to replicate autonomously. Knowledge of viral genome release may provide understanding of viral replication and a method to artificially inactivate viral particles. Currently, little is known about the release model of porcine circovirus type 2 (PCV2). Here, we report the release of the PCV2 genome from assembled capsid and the intracellular trafficking of infectious PCV2 by alterations in the capsid conformation. Knowledge of PCV2 capsid stability and dynamics is essential to understanding its infectious cycle and lays the foundation for discovering powerful targets for therapeutic and prophylactic intervention.

scholarly journals Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I

2018 ◽  
Vol 63 (No. 8) ◽  
pp. 358-366
Author(s):  
LL Zheng ◽  
XH Jin ◽  
FS Wei ◽  
CQ Wang ◽  
HY Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pseudorabies Virus ◽  
Simultaneous Detection ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Porcine Parvovirus ◽  
Porcine Pseudorabies Virus

Porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 can cause reproductive failure in pigs, and swine are often simultaneously infected by combinations of the three viruses. We here report the development of a SYBR Green I-based multiplex real time PCR assay for simultaneous detection of porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2. Three pairs of specific primers were designed for the porcine parvovirus-VP2, porcine pseudorabies virus-gH and porcine circovirus type 2-ORF2 genes. Viral genomes were identified based on their distinctive melting temperatures in singleplex PCR reactions. The melting temperature was 74.5 °C for the 313 bp amplicon of porcine parvovirus-VP2 gene, 87.5 °C for the 355 bp amplicon of porcine pseudorabies virus-gH gene and 80.5 °C for the 171 bp amplicon of the porcine circovirus type 2-ORF2 gene, respectively. The detection limit of the method ranged from 0.01–0.03 TCID<sub>50</sub>/ml for the three viruses. In addition, porcine parvovirus, porcine pseudorabies virus and porcine circovirus type 2 viral loads were measured in 100 field samples, and the result showed that the concordance between real-time PCR and conventional PCR was 60.42%. The sensitivity and specificity of real-time PCR were 100% and 100%, while those of conventional PCR were 40.83% and 72.22%, respectively.

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