Single dilution Avidity-Blocking ELISA as an alternative to the Bovine Viral Diarrhea Virus neutralization test

2011 ◽  
Vol 175 (2) ◽  
pp. 228-235 ◽  
Author(s):  
O.L. Franco Mahecha ◽  
M.L. Ogas Castells ◽  
G. Combessies ◽  
M.A. Lavoria ◽  
M. Wilda ◽  
...  
2014 ◽  
Vol 95 (7) ◽  
pp. 1522-1531
Author(s):  
Mandy Gebauer ◽  
Martina Behrens ◽  
Matthias König ◽  
Sven-Erik Behrens

Infections with bovine viral diarrhea virus (BVDV) have a huge economic impact on cattle production and reproduction worldwide. A key factor for BVDV surveillance and eventual eradication is to efficiently detect infections and to monitor herd immunity. In this study, we generated a stable, bi-cistronic BVDV that encoded EGFP in addition to the viral proteins. Applying this recombinant virus, a new flow-cytometry-based virus neutralization test was established that enabled accurate and reliable detection of field-virus-infected and vaccinated animals. The test, which is simple and fast, is expected to support novel, effective screening procedures in eradication and vaccination programmes.


Author(s):  
Henrique Meiroz de Souza Almeida ◽  
Igor Renan Honorato Gatto ◽  
Karla Alvarenga Nascimento ◽  
Marina Lopes Mechler Dreibi ◽  
Samir Issa Samara ◽  
...  

2004 ◽  
Vol 64 (2) ◽  
pp. 113-118 ◽  
Author(s):  
M GIVENS ◽  
D STRINGFELLOW ◽  
C DYKSTRA ◽  
K RIDDELL ◽  
P GALIK ◽  
...  

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