Multiplex allele-specific PCR combined with PCR-RFLP analysis for rapid detection of gyrA gene fluoroquinolone resistance mutations in Mycobacterium tuberculosis

2012 ◽  
Vol 88 (1) ◽  
pp. 175-178 ◽  
Author(s):  
Li-li Zhao ◽  
Qiang Xia ◽  
Nan Lin ◽  
Zhi-guang Liu ◽  
Xiu-qin Zhao ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Rflp Analysis ◽  
Fluoroquinolone Resistance ◽  
Resistance Mutations ◽  
Gyra Gene ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Allele Specific Pcr

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

Rapid Detection of Factor XIII Val34Leu by Allele Specific PCR

1999 ◽  
Vol 81 (03) ◽  
pp. 463-463 ◽  
Author(s):  
M. Henry ◽  
P. E. Morange ◽  
I. Canavy ◽  
M. C. Alessi ◽  
I. Juhan-Vague
Keyword(s):  
Rapid Detection ◽  
Factor Xiii ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Design of Allele Specific PCR for Rapid Detection of CYP3A5 (A6986G) and Mdr-1 (C3435T) Polymorphisms

2010 ◽  
Vol 26 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Tester F. Ashavaid ◽  
Himanshu S. Raje ◽  
Bharat V. Shah ◽  
Swarup A. Shah
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Mdr 1

scholarly journals An Allele-Specific PCR System for Rapid Detection and Discrimination of the CYP2C19∗4A, ∗4B, and ∗17 Alleles

2013 ◽  
Vol 15 (6) ◽  
pp. 783-789 ◽  
Author(s):  
Stuart A. Scott ◽  
Qian Tan ◽  
Usman Baber ◽  
Yao Yang ◽  
Suparna Martis ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr
Sign in / Sign up

Export Citation Format

Share Document