scholarly journals A Whole-Brain Atlas of Inputs to Serotonergic Neurons of the Dorsal and Median Raphe Nuclei

Neuron ◽  
2014 ◽  
Vol 83 (3) ◽  
pp. 663-678 ◽  
Author(s):  
Iskra Pollak Dorocic ◽  
Daniel Fürth ◽  
Yang Xuan ◽  
Yvonne Johansson ◽  
Laura Pozzi ◽  
...  
Keyword(s):  
Raphe Nuclei ◽  
Brain Atlas ◽  
Median Raphe ◽  
Serotonergic Neurons ◽  
Whole Brain ◽  
Raphé Nuclei ◽  
Median Raphé

scholarly journals Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Zhengchao Xu ◽  
Zhao Feng ◽  
Mengting Zhao ◽  
Qingtao Sun ◽  
Lei Deng ◽  
...  
Keyword(s):  
Brain Regions ◽  
Gabaergic Neurons ◽  
Raphe Nucleus ◽  
Raphe Nuclei ◽  
Brain Atlas ◽  
Median Raphe ◽  
Whole Brain ◽  
Lateral Habenula ◽  
Raphé Nuclei ◽  
Median Raphé

The dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the DR and MR. We found that these neurons received inputs from similar upstream brain regions. The glutamatergic and GABAergic neurons in the same raphe nucleus had divergent projection patterns with differences in critical brain regions. Specifically, MR glutamatergic neurons projected to the lateral habenula through multiple pathways. Correlation and cluster analysis revealed that glutamatergic and GABAergic neurons in the same raphe nucleus received heterogeneous inputs and sent different collateral projections. This connectivity atlas further elucidates the anatomical architecture of the raphe nuclei, which could facilitate better understanding of their behavioral functions.

scholarly journals Author response: Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei

2019 ◽  
Author(s):  
Jing Ren ◽  
Alina Isakova ◽  
Drew Friedmann ◽  
Jiawei Zeng ◽  
Sophie M Grutzner ◽  
...  
Keyword(s):  
Single Cell ◽  
Raphe Nuclei ◽  
Author Response ◽  
Median Raphe ◽  
Whole Brain ◽  
Serotonin Neurons ◽  
Raphé Nuclei ◽  
Median Raphé

scholarly journals Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Jing Ren ◽  
Alina Isakova ◽  
Drew Friedmann ◽  
Jiawei Zeng ◽  
Sophie M Grutzner ◽  
...  
Keyword(s):  
Single Cell ◽  
Glutamate Transporter ◽  
Raphe Nuclei ◽  
Molecular Heterogeneity ◽  
Median Raphe ◽  
Axonal Projection ◽  
Whole Brain ◽  
Serotonin Neurons ◽  
Raphé Nuclei ◽  
Median Raphé

Serotonin neurons of the dorsal and median raphe nuclei (DR, MR) collectively innervate the entire forebrain and midbrain, modulating diverse physiology and behavior. To gain a fundamental understanding of their molecular heterogeneity, we used plate-based single-cell RNA-sequencing to generate a comprehensive dataset comprising eleven transcriptomically distinct serotonin neuron clusters. Systematic in situ hybridization mapped specific clusters to the principal DR, caudal DR, or MR. These transcriptomic clusters differentially express a rich repertoire of neuropeptides, receptors, ion channels, and transcription factors. We generated novel intersectional viral-genetic tools to access specific subpopulations. Whole-brain axonal projection mapping revealed that DR serotonin neurons co-expressing vesicular glutamate transporter-3 preferentially innervate the cortex, whereas those co-expressing thyrotropin-releasing hormone innervate subcortical regions in particular the hypothalamus. Reconstruction of 50 individual DR serotonin neurons revealed diverse and segregated axonal projection patterns at the single-cell level. Together, these results provide a molecular foundation of the heterogenous serotonin neuronal phenotypes.

Differential behavioral and neurochemical effects following lesions of the dorsal or median raphe nuclei in rats

1974 ◽  
Vol 79 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Barry L. Jacobs ◽  
William D. Wise ◽  
Kenneth M. Taylor
Keyword(s):  
Raphe Nuclei ◽  
Median Raphe ◽  
Raphé Nuclei ◽  
Median Raphé

scholarly journals Effects of water restriction on social behavior and 5-HT neurons density in the dorsal and median raphe nuclei in mice

2021 ◽  
Vol 399 ◽  
pp. 113022
Author(s):  
Houari Boukersi ◽  
Nemcha Lebaili ◽  
Anne Nosjean ◽  
Nathalie Samson ◽  
Alexis Faure ◽  
...  
Keyword(s):  
Social Behavior ◽  
Raphe Nuclei ◽  
Median Raphe ◽  
Water Restriction ◽  
Raphé Nuclei ◽  
Median Raphé
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