Purification and functional characterization of tomato mosaic virus 130K protein expressed in silkworm pupae using a baculovirus vector

Abstract The present study reports observation of a field chilli pepper disease consisting of a co-infection with two potyviruses: Wild tomato mosaic virus Dehong isolate (WTMV-Dh) and Chili veinal mottle virus Dehong isolate (ChiVMV-Dh). We obtained the complete genome sequences of these two viruses by NGS sequencing. The WTMV-Dh is 9,598 nucleotides (nt) in length and encodes a complete polyprotein of 3,075 amino acids (aa). The polyprotein of WTMV-Dh shares 76.1–82.6% nt and 85.3–89.5% aa identities with the other three WTMV isolates reported previously. The ChiVMV-Dh is 9688 nt in length and encodes a complete polyprotein with 3, 089 aa. The polyprotein of ChiVMV-Dh shares 80.8–92.2% nt and 85.3–95.6% aa identities with the other ChiVMV isolates reported previously. Following phylogenetic analysis based on the polyprotein sequences of other potyviruses, WTMV-Dh clustered with the Vietnam strain WTMV-Laichau while ChiVMV-Dh clustered with several ChiVMV Sichuan isolates. Evaluation of the recombination events within the WTMV and ChiVMV subgroups indicated that some putative recombination events occurred in critical regions. These regions include the N-terminal of HC-Pro and P1 region of WTMV-Dh, CP and the P3 to CI region of ChiVMV-Dh, which may be new evidence of adaptive evolution of potyviruses.

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v1 ◽

2019 ◽

Author(s):

Mario Houde ◽

Arnaud Cheuk ◽

François Ouellet

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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Characterization of tomato mosaic virus and search for its resistance in Solanum species

European Journal of Plant Pathology ◽

10.1007/s10658-019-01848-2 ◽

2019 ◽

Vol 155 (4) ◽

pp. 1195-1209

Author(s):

Najeeb Ullah ◽

Khalid Pervaiz Akhtar ◽

Muhammad Yussouf Saleem ◽

Mudasser Habib

Keyword(s):

Mosaic Virus ◽

Solanum Species ◽

Tomato Mosaic Virus

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v2 ◽

2020 ◽

Author(s):

Arnaud Cheuk ◽

François Ouellet ◽

Mario Houde

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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Characterization of a specific interaction between IP-L, a tobacco protein localized in the thylakoid membranes, and Tomato mosaic virus coat protein

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2008.07.010 ◽

2008 ◽

Vol 374 (2) ◽

pp. 253-257 ◽

Cited By ~ 18

Author(s):

Chaozheng Zhang ◽

Yueyong Liu ◽

Xianchao Sun ◽

Weidong Qian ◽

Dongdong Zhang ◽

...

Keyword(s):

Coat Protein ◽

Mosaic Virus ◽

Specific Interaction ◽

Thylakoid Membranes ◽

Tomato Mosaic Virus ◽

Virus Coat Protein ◽

Virus Coat

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v3 ◽

2020 ◽

Author(s):

Arnaud Cheuk ◽

François Ouellet ◽

Mario Houde

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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