barley stripe mosaic virus
Recently Published Documents


TOTAL DOCUMENTS

207
(FIVE YEARS 14)

H-INDEX

32
(FIVE YEARS 1)

2022 ◽  
Author(s):  
Zhihao Jiang ◽  
Xuejiao Jin ◽  
Meng Yang ◽  
Qinglin Pi ◽  
Qing Cao ◽  
...  

Salicylic acid (SA) acts as a signaling molecule to perceive and defend against pathogen infections. Accordingly, pathogens evolve versatile strategies to disrupt the SA-mediated signal transduction. However, it is necessary to further characterize how plant viruses manipulate the SA-dependent defense responses. Here, we show that Barley stripe mosaic virus (BSMV) infection activates SA-mediated defense signaling pathway and upregulates the expression of Nicotiana benthamiana thioredoxin h-type 1 (NbTRXh1). The γb protein interacts directly with NbTRXh1 in vivo and in vitro. Overexpression of NbTRXh1, but not a reductase-defective mutant, impedes BSMV infection, whereas low NbTRXh1 expression level results in increased viral accumulation. Similar with its orthologues in Arabidopsis, NbTRXh1 also plays an essential role in SA signaling transduction in N. benthamiana. To counteract NbTRXh1-mediated defenses, the BSMV ?b protein targets NbTRXh1 to dampen its reductase activity and thereby impairing downstream SA defense genes expression to optimize viral cell-to-cell movement. We also found that NbTRXh1-mediated resistance defends against Lychnis ringspot virus, Beet black scorch virus, and Beet necrotic yellow vein virus. Taken together, our results reveal a novel role for the multifunctional γb protein in counteracting plant defense responses, and broadens the broad-spectrum antibiotic role of SA signaling pathway.


2021 ◽  
Author(s):  
Malgorzata Jezewska

Abstract In the USA, BSMV has been economically significant only in Montana and North Dakota (Carroll, 1983). Between 1953 and 1970 the total loss in barley caused by this virus was calculated at more than US$ 30 million. In 1964 the virus caused damage estimated at over US$ 3.1 million (Carroll, 1980). Yield reductions in barley were 24-46% when BSMV was inoculated mechanically (Chiko and Barker, 1978; Carroll, 1980). In most states of the USA and also in most other countries where it occurs, the economic significance of BSMV has decreased (Carroll, 1983). For further information, see Carroll (1986). The impact of mild strains of BSMV on barley yield is substantially lower (Jezewska, 2006).


2020 ◽  
Vol 3 (12) ◽  
pp. 12080-12086
Author(s):  
Yu-Hsuan Lee ◽  
Kok Zhi Lee ◽  
Rachel G. Susler ◽  
Corren A. Scott ◽  
Longfei Wang ◽  
...  

2020 ◽  
Vol 16 (7) ◽  
pp. e1008709 ◽  
Author(s):  
Zhihao Jiang ◽  
Kun Zhang ◽  
Zhaolei Li ◽  
Zhenggang Li ◽  
Meng Yang ◽  
...  

2020 ◽  
Author(s):  
Kok Zhi Lee ◽  
Vindula Basnayake Pussepitiya ◽  
Yu Hsuan Lee ◽  
Sue Loesch Fries ◽  
Michael Harris ◽  
...  

2020 ◽  
Author(s):  
Arnaud Cheuk ◽  
François Ouellet ◽  
Mario Houde

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.


2020 ◽  
Author(s):  
Arnaud Cheuk ◽  
François Ouellet ◽  
Mario Houde

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.


Sign in / Sign up

Export Citation Format

Share Document