In vitro production and chemical characterization of St. John’s wort (Hypericum perforatum L. cv ‘New Stem’)

Plant Science ◽  
2004 ◽  
Vol 166 (2) ◽  
pp. 333-340 ◽  
Author(s):  
S.M.A. Zobayed ◽  
S.J. Murch ◽  
H.P.V. Rupasinghe ◽  
P.K. Saxena
2017 ◽  
Vol 20 ◽  
pp. 470-480 ◽  
Author(s):  
Miriam Heinrich ◽  
Veronika Vikuk ◽  
Rolf Daniels ◽  
Florian C. Stintzing ◽  
Dietmar R. Kammerer

2011 ◽  
Vol 72 (14-15) ◽  
pp. 1814-1820 ◽  
Author(s):  
Satomi Onoue ◽  
Yoshiki Seto ◽  
Masanori Ochi ◽  
Ryo Inoue ◽  
Hideyuki Ito ◽  
...  

2004 ◽  
Vol 21 (4) ◽  
pp. 453-463 ◽  
Author(s):  
Christoph Seger ◽  
Harald Römpp ◽  
Sonja Sturm ◽  
Ernst Haslinger ◽  
Peter C Schmidt ◽  
...  

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 978-980 ◽  
Author(s):  
Joe-Ann McCoy ◽  
N.D. Camper

Hypericum perforatum L. (St. John's Wort) has an extensive history as an important medicinal herb used for the treatment of neurological and depressive disorders (Linde et al., 1996). The objective of this study was to establish an in vitro tissue culture protocol for St. John's Wort. Nodal segments, axillary buds, and leaf disc explants produced multiple shoots and callus on Murashige and Skoog minimal organics medium supplemented with combinations of indoleacetic acid (IAA; 0.57, 2.85, 5.71 μm) and benzylaminopurine (BA; 2.22, 4.44, 8.88 μm). Shoot production occurred on all combinations of IAA/BA tested and was significantly less in treatments without hormones. Callus production was higher on treatments containing 2.85 μm IAA + 4.44 μm BA, or 5.71 μm IAA + 8.88 μm BA. Shoots transferred to hormone-free medium at 8 weeks formed roots by 12 weeks. A micropropagation protocol was established for St. John's Wort using mature plants as the explant source.


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